Kanupriya Kusumakar
Maulana Azad Medical College-Lok Nayak Hospital, India
Title: Bacteriological profile of patients attending infertility clinic
Biography
Biography: Kanupriya Kusumakar
Abstract
Statement of the Problem: Urogenital infection can play an important role in the etiology of infertility. Such infections may often go unnoticed but could hamper infertility. These infections may affect fertility in several ways: By damaging sperm, hampering their motility, altering the chemical composition of the seminal fluid or by producing an inflammatory structure in the tract. The purpose of this study was to identify the bacterial isolates by conventional methods in patients attending infertility clinic.
Methodology & Theoretical Orientation: A total of 250 semen samples and 150 cervical samples were examined. The samples were inoculated on blood agar plates containing 5% sheep blood agar and MacConkey’s agar plates. The plates were incubated at 37 oC with 5% CO2 for 24 hours and examined. Biochemical tests were used for identification and confirmation of the bacterial isolates.
Findings: In our study, bacterial isolates were seen in 112 (44.8%) of the 250 semen samples and 77 (51.33%) of 150 cervical samples examined. The major organisms isolated from semen samples were Pseudomonas sp. 41/250 (16.4%), Escherichia coli 29/250 (11.6%) and Staphylococcus sp. 24/250 (9.6%). The main organisms isolated from cervical samples were Escherichia coli 12/150 (8.00%), Klebsiella sp. 9/150 (6.00%) and Acinetobacter sp. 3/150 (2.00%).
Conclusion & Significance: It is necessary to determine not just the presence of bacteria but also their nature as well as numbers while evaluating an infertile patient. It would be worthwhile to screen routinely all patients for a possible silent urogenital tract infection attending infertility clinic and vigorously institute appropriate antibacterial therapy wherever indicated by significant culture.