Zhansheng Wu has completed his PhD at 2011 from Beijing Institute of Technology University. He is the director of Yanhui, is a doctor student from Shihezi University and visiting scholar from Colorado State University Horticulture and Landscape Architecture. He has published more than 50 papers in reputed journals and has been serving as a reviewer board member of repute.
The present study was designed with the objective to analyze the effect of Pseudomonas putida Rs-198 (Rs-198) on phosphorus (P) content and bacteria communities of agricultural soils and nutrient accumulation in pepper. The application of Rs-198 did have a positive effect on available P content in bulk and rhizosphere soils, the available P content significantly increased from 51.63 to 83.54 μg/kg after Rs-198 inoculation. Significant differences in microbial composition and predicted metagenome were observed between the Rs-198 inoculation treatments compared to the control. This effect was driven by differences in the relative abundances of P content and soil pH associated with Rs-198 application. Principal coordinates analysis (PCoA) showed that the Rs-198 inoculation had more correlation patterns between dominant bacteria taxa and important microbes that contribute to soil nutrition cycling. Overall, Rs-198 inoculation increased N, P and K contents in pepper stem by 18.40%, 25.00% and 25.07% respectively. Additionally, Rs-198 inoculation significantly decreased Na content in pepper root by 59.71%. Inoculation of Rs-198 showed promotion effects on biomass of pepper, which may also contribute to the increase of soil available P content and beneficial changes of key nutrition cycling microorganisms. These findings indicate that the beneficial changes in soil environment condition, such as soil nutrition, bacteria community and functional bacteria diversity could be attributed to inoculation of Rs-198 communities.
Msolo Luyanda has completed his Master degree at the age of 24 years from the University of Fort Hare and is currently enrolled for his Doctoral degree (final year) in Microbiology. He is a full member of the Applied and Environmental Microbiology Research Group (AEMREG) and South African Medical Research Council- Water Quality Monitoring Centre . He is currently working on publishing more than 3 papers from his Ph. D Research work. He is a disciplined, intelligent and diligent male with lots of potential. My enthusiasm, capabilities and abilities are that of an aspiring
Escherichia coli O157:H7 is one of the most imperious foodborne pathogens predisposed for a number of mortalities worldwide. To investigate the occurrence and antibiotics susceptibility of Escherichia coli (E. coli) from three selected commercial dairy farms in the Amathole District Municipality, Eastern Cape Province, South Africa, raw milk samples were collected from bulk storage tanks and swab samples from milking machines, cattle udder(s) and workers hands were also collected on a six-month sampling regime between June and November 2014. A standard culture-based method was used for the enumeration and isolation of E. coli O157:H7 using sorbitol MacConkey agar (supplemented with cefixime (50 μg/L) and potassium tellurite (25 mg/L). A serological confirmation of the presumptive E. coli O157:H7 isolates was conducted using the O157 latex agglutination test kit. A total of 252 E. coli O157:H7 isolates were further subjected to PCR detection of rfbE O157 and fliCH7 genes of which 27(11%) of the isolates were confirmed positive E. coli O157:H7. Our finding reveal that of the 27 E. coli O157:H7 isolates from the dairy farms, rate of resistance against penicillin was 85% and resistance against the other antibiotics follow the order: tetracycline (81%), erythromycin (70%), streptomycin (52%) and chloramphenicol (45%). We conclude that the dairy farms are potential reservoirs of E. coli O157:H7 serotype with multiple antibiotic resistance and consequently a concern to public and environmental health.