Dilip Kumar
Singapore Immunology Network, Singapore
Title: The genetic variants control resistin expression through promoter suppression which also leads to the modulation of resistin associated disease pathways
Biography
Biography: Dilip Kumar
Abstract
Resistin is a member of adepokine family of cytokines and is known to modulate a diverse set of chemokines and cytokines. In humans, resistin is primarily produced by immune cell other than adipocytes which is the major source in mouse. It has been widely studied in the context of human inflammatory and metabolic diseases. The putative role of resistin in the pathogenesis of human diseases led to several genetic studies in different populations. Resistin is well known to be regulated by Cis- regulatory variants and also led to the predisposition towards diseases, but recognizing the precise targets, mechanisms and biological implications of resistin associated cis-regulatory variants are still poorly understood.
Based on whole blood, cell type specific eQTLs, resistin promoter reporter analysis, EMSA, inhibition study and bisulphite sequencing, we propose that the suppression of resistin promoter is the underlying mechanisms for genetic regulation of resistin expression in monocytes. Also a genetically defined Cohort based immune-phenotyping and plasma biomarkers analysis showed a significant modulation of immune cells and plasma biomarker which are known to be associated with inflammatory diseases.
Our study could provide detailed understanding about the role of resistin associated genetic cis-regulatory variants in the context inflammatory diseases and leads to the better identification and design of new therapeutic targets in the field of inflammation.