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12th Annual Meet on Bacteriology & Applied Microbiology

Singapore City, singapore

Ilknur Tuncer,

Ilknur Tuncer,

Dokuz Eylul University, Turkey

Title: Phylogenetic diversity, antibiotic susceptibility and enzymatic activities of bacterial isolates from sediments of Eastern Mediterranean Sea

Biography

Biography: Ilknur Tuncer,

Abstract

Phylogenetic diversity of isolated bacteria and biogeochemical variability of sediments in shallow and deep basins of Eastern Mediterranean Sea were studied. According to 16S rRNA gene sequence analysis, the strains isolated from the study area were belonged to the Firmicutes, Gammaproteobacteria and Actinobacteria. The present study provided highly diverse bacterial strains of 14 different families especially with probability of representing new taxa in addition to the dominant genus bacillus in each sediment samples. The actinomycetes were tested physiologically using commercial kits and found
that they utilized proteins rather than carbohydrates. Geochemical parameters of the sediments together with enzymatic activity results of the strains underlined the nitrogen limitation in the area. The highest antibiotic resistance for the Firmicutes and Gammaproteobacteria strains was obtained from coastal sediments compared to deep basins supported by a positive correlation between sediment size and antibiotic resistance. The influence of environmental parameters was observed as the variability of bacterial community composition such that phylogenetic diversity was much higher in higher taxa for shallower
regions, especially in North Aegean Sea as compared to deep basins of Eastern Mediterranean Sea as supported in hierarchical cluster analysis of the isolates and statistical analysis including principle component analysis.