Murtala Saadu
Kebbi State University of Science and Technology, Nigeria
Title: synergistic effect of alkaloid and saponin of synergistic effect of ALKALOID and SAPONIN of Vernonia amygdalina on Escherichia coli
Biography
Biography: Murtala Saadu
Abstract
The study was aimed at investigate quantitatively phytochemical constituents of the leaf extract of Vernonia amygdalina, and determine the Synergistic effect of Alkaloid and Saponin compound of the plant extract against E. coli. The antibacterial activities of the alkaloid, saponin and their synergistic effect were carried out at varying concentration of 50, 25, 12.5 and 6.25mg/ml, using agar well diffusion method. The result of this study indicated the, ethanolic extract shows higher anti-bacterial activity at 100mg/ml concentration (11.29mm) compared to aqueous extract (9.24mm) against the test bacteria. It also showed that the anti-bacterial activity of the alkaloid content of the Vernonia amygdalina at 100mg/ml (11.01mm) was better than that of saponin (9.32mm) while the synergistic effect of alkaloid and saponin at concentration 100mg/ml (15.41mm). Were better than the individual compounds the result indicates the possibility of using the alkaloid and saponin compounds synergistically in the treatment of disease associated with E.coli.