Zartasha Zafar
Pir Mehr Ali Shah Arid Agriculture University, Pakistan
Title: Bacterial flora of Indian red jungle fowl (Gallus gallus murghi) semen and their sensitivity profile
Biography
Biography: Zartasha Zafar
Abstract
Presence of bacteria in semen ejaculate is detrimental to sperm survival. The present study was designed to isolate, identify bacteria in the semen ejaculate, their antibiotic sensitivity and the effect of antibiotics on post-thaw semen quality of Indian red jungle fowl (Gallus gallus murghi). Semen was collected from eight cocks of Indian red jungle fowl and split into 6 treatments having Streptomycin, Neomycin, Kanamycin, Gentamicin, Penicillin and control (Without antibiotic). The sample was cryopreserved stored in liquid nitrogen for further processing. Sperm Motility, Plasma Membrane Integrity, livability and Acrosomal Integrity were observed at different stages of cryopreservation
(Post-dilution, post-cooling, post-equilibration and post-thawing).. The antibiotic addition to semen extender did not pose any significant affect (P>0.05) on semen quality parameters as compared to control. The bacterial contaminants isolated were Escherichia Coli, Staphylococcus spp. and Bacillus spp. All of these bacteria were found sensitive to Penicillin, Neomycin and Kanamycin. Staphylococcus spp were sensitive to streptomycin, Penicillin and Neomycin. While Bacillus spp was found sensitive to Penicillin and Gentamicin. It was concluded that addition of antibiotics to semen extenders did not cause any toxicity and maintained semen quality as that of untreated control samples. It was found that Penicillin was the most effective antibiotic in the present study. Therefore it was recommended that Penicillin can be added to semen extenders for the control of bacterial contamination without affecting semen quality of Indian red jungle fowl.