Scientific Program

Conference Series Ltd invites all the participants across the globe to attend 7th International Conference and Exhibition on Bacteriology & Antibiotics Vancouver, Canada.

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bacteriology@annualamericacongress.org
antibiotics@annualamericacongress.com
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Scientific Program Day 1
Scientific Program Day 2

Day 2 :

Keynote Forum

Minerva A Garcia

Jacobi Medical Center, USA

Keynote: Difficulties encountered in the isolation of Streptobacillus moniliformis, a GVPmBdCurvB
Conference Series Bacteriology 2019 International Conference Keynote Speaker Minerva A Garcia photo
Biography:

Minerva A. Garcia is an Asscoiate Director Microbiology at Jacobi Medical Center in NYC. She has published three papers in major reputable journals has has presented more than 10 abstracts via poster presenations included Harvard Medical School New Research Building sponsored by Society of Biomolecular Imaging and Infomatics Second Annual Conference among many others. Currently, thesis publication “Evaluation of Vancomycin MICs for the Treatment of MRSA  in Bacteremia”. She has been involved with American Society of Microbiology national and regional for more than thirty years.

 

Abstract:

A blood culture isolate of an unusual morphology appearing alpha colonies of facultative anaerobe, GV-PmBdCurvB (Gram Variable Pleomorphic Beaded Curved Bacillus) microorganism was isolated from an aerobic blood culture of a 51-year-old male that was presented to ER with temperature of 39oC. Possibly seems that after being found in the subway floor possibly bitten by a rat in the subway and/or eaten contaminated food.  This was one of the findings as per the identification of the microbe. The patient presented with myalgia, arthralgia and chills. The blood culture taken turned positive after day 3. It was returned to the instrument because was non-viable and failed to grow within 48 hours aerobically, anaerobically and microaerophilic. Gram stain revealed a Gram variable bacilli 3% KOH was used to determine its gram variability as a guide to properly guide the clinician. Upon further examination, on day 5, it revealed the microbe was fastidious pathogen with morphology of G + C content growing and requiring 5 to 7 days of further incubation. This organism thought was mixed with two different microbes, turned out that it was not. It was the same strain. It proved to have distinctive features: Gram variable pleomorphic beaded/branching and curved bacilli. It was suspected as two distinctive microorganisms (small and large colonies) identified as one. It failed automation identification as our lab test showed inert biochemically and was forwarded to our Public Health reference lab- NYCDOHMH, they were unable to identify and thus forwarded to NYSDOHWC. NYSDOHWC Identified as S. moniliformis by culture, biochemicals and Bacterial DNA Sequence Analysis. The patient was treated with Vanco-mycin 1g q12 and Meropenem 1g q8 and responded well. The organism took closely to two months for identification due to slow growth characteristic likely the reason and its and gram variable.

NYCDOHMH (NYC DOH Public Health laboratory)/NYSDOHWC (NYS DOH Wadsworth Center)

 

Keynote Forum

Samir Jaoua

Qatar University, Qatar

Keynote: Applications of heterologous gene expression in Bacillus thuringiensis, Photorhabdus luminescens and Escherichia coli for the overproduction of metabolites

Time : 11:15-12:10

Conference Series Bacteriology 2019 International Conference Keynote Speaker Samir Jaoua photo
Biography:

Samir Jaoua, has completed his PhD at the age of 27 years from the University of Technology of Compiegne (UTC, France), and postdoctoral studies from Ciba-Geigy (Novartis) in Basle (Switzerland). He is Professor of Microbiology at the Department of Biological and Environmental Sciences (CAS, Qatar Univ.). He is also Qualified Professor of France Universities “Section 64: Molecular Biology and Biochemistry” and Professor at the University of Sfax (Tunisia). Prof. Samir Jaoua is molecular and microbial geneticist. He has published 126 papers in reputed journals. His H-index is 32 with 3125 citations.

Abstract:

Bacillus thuringiensis (Bt) is a Gram positive bacterium characterised by the production, during sporulation, of various toxins having insecticidal activities, among which the delta-endotoxins Cry and the vegetative insecticidal proteins Vip. Hundreds of strains of B. thuringiensis, isolated from Tunisia, Qatar and other countries were studied and their bioinsecticides coding genes cry and vip, were cloned and characterized. Among the Tunisian strains, we evidenced the abundance of the kurstaki subspecies active on Lepidoptera and particularly the lepidopteran olive tree pathogenic insect P. oleae, whereas from the Qatari soil samples, we evidenced the abundance of the spherical crystal producing strains, among which subspecies active on Diptera and disease vetors. The bacterium Bacillus thuringiensis produces, at the vegetative stage of its growth, Vip3A proteins with activity against a broad spectrum of lepidopteran insects. We performed the heterologous expression of corresponding coding genes in B. thuringiensis, P. luminescens and E. coli and evidenced an increase of the synthesis of the insecticidal proteins. B. thuringiensis produces also antifungal chitinases. One chitinase was characterized by both its high chitinolytic and antifungal activities. The cloning and sequencing of the corresponding gene chi255, showed is a new chitinase Chi255, presenting several differences from the published chitinases of B. thuringiensis. Heterologous expression in E. coli was performed by cloning the chi255 ORF downstream a strong promoter in the vector pBAD. Identification, by HPLC analysis, of chitin hydrolysis products issued from the activity exhibited by Chi255, revealed that this enzyme is a chitobiosidase. By heterologous expression we succeeded in integrating the enzyme in the B. thuringiensis crystals.