Scientific Program

Conference Series Ltd invites all the participants across the globe to attend 12th Annual Meet on Bacteriology & Applied Microbiology Singapore.

Day 2 :

  • Clinical Diagnostic Bacteriology | Microbial Genomics | Applied Microbiology | Medical Microbiology | Dairy Microbiology | Aquatic Microbiology
Speaker
Biography:

Prof. Andrzej Lange, MD, FRCP graduated with a medical degree with distinction from the Medical School in Wrocław, Poland is a professor in the Institute of Immunology and Exp Therapy of the Polish Academy of Sciences and a founder and head of the Lower Silesian Center of Cellular Transplantation in Wrocław. His international experience started in 1973 – 1974 as a Leverhulme Fellow in the Middlesex Hospital Medical School, London. He has been a visitor and lectured in a number of European and North American scientific institutions. Known from his activity in the field of bone marrow transplantation and regenerative medicine.  

Prof. Lange was awarded with several scientific distinctions and served to a number of National and European institutions being also active in co-editing journals.  He is an author and co-author of 249 scientific papers, in peer-reviewed journals with a cumulative IF of 270 in the years 1995–2017

Abstract:

In 2004 we started a project on revascularization of legs with critical ischemia (CLI). Eleven years later a similar approach was employed to improve vascularization of the femur with avascular necrosis (AVN). In both attempts a leukophoretic product resulting from the separation (Spectra, Optia) of 500 mL of the marrow was injected in small portions into the calf of legs with ischemia or into Kirschner wire drilled holes penetrating the head of the femur.  In CLI patients employed procedure resulted in a prompt relief of pain followed by healing of the ulceration and significantly prolonged intervals between episodes of intermittent claudication. Forty six percent of patients benefited from the improvement. As to AVN, an improvement seen in 3 out of 5 patients was reported as pain relief and improvement in movement. 
More recently instead of leukophoresis, a commercially available kit was used for the marrow manipulation. The cells were injected into the hip (7 cases) or the knee (12 cases). The effect was seen as soon as two weeks after the implantation as the relief of pain and improvement in movement. 
A higher contribution of CD45-CD34- CD90+ and CD73+ cells to the implanted inoculum was beneficial for osteoarthritis treatment but not in improvement of vasculature (likely endothelial stem cells). In the inoculum used for regeneration two subpopulations of cells were identified having endothelial or mesenchymal cell markers. Notably the cells having an unique marker and that of stemness potential, were localized in the gate of small forward and side scatters.  
In conclusion, the angiogenic and anti-inflammatory potential of BMMNC has been clinically documented, and the information that different subsets of the cellular inoculum contribute to the clinical outcome opens a new avenue for targeted therapy using subsets of stem cells for optimal application of treatment
 

Alain Chapel

Institute of Radiological Protection and Nuclear Safety, France

Title: Stem cell therapy for the treatment of severe tissue damage after radiation exposure
Speaker
Biography:

For 25 years, he has been developing gene and cell therapy using non-human primates, immune-tolerant mice and rats to protect against the side effects of radiation. He collaborates with clinicians to develop  strategies for treatment of patients after radiotherapy overexposures. He has participated in the first establishment of proof of concept of the therapeutic efficacy of Mesenchymal stem cells (MSCs) for the treatment of hematopoietic deficit, radiodermatitis and over dosages of radiotherapy. He has contributed to the first reported correction of deficient hematopoiesis in patients (graft failure and aplastic anemia) thanks to intravenous injection of MSCs restoring the bone marrow microenvironment, mandatory to sustain hematopoiesis after total body irradiation. He is scientific investigator of Clinical phase II trial evaluating the efficacy of systemic MSC injections for the treatment of severe and chronic radiotherapy-induced abdomino-pelvic complications refractory to standard therapy (NCT02814864Hirsch Index 26.

Abstract:

The late adverse effects of pelvic radiotherapy concern 5 to 10% of them, which could be life threatening. However, a clear medical consensus concerning the clinical management of such healthy tissue sequelae does not exist. Our group has demonstrated in preclinical animal models that systemic MSC injection is a promise approach for the medical management of gastrointestinal disorder after irradiation. We have shown that MSC migrate to damaged tissues and restore gut functions after irradiation.

The clinical status of four first patients suffering from severe pelvic side effects resulting from an over-dosage was improved following MSC injection in a compassional situation. A quantity of 2x106 - 6x106 MSC /kg were infused intravenously to the patients. Pain, hemorrhage, frequency of diarrheas and fistulisation as well as the lymphocyte subsets in peripheral blood were evaluated before MSC therapy and during the follow-up. Two patients revealed a substantiated clinical response for pain and hemorrhage after MSC therapy. In one patient pain reappeared after 6 months and again substantially responded on a second MSC infusion. A beginning fistulisation process could be stopped in one patient resulting in a stable remission for more than 3 years of follow-up. The frequency of painful diarrhea diminished from an average of 6/d to 3/d after the first and 2/d after the 2nd MSC injection in one patient. In all patients, prostate cancer remained in stable complete remission. A modulation of the lymphocyte subsets towards a regulatory pattern and diminution of activated T cells accompanies the clinical response in refractory irradiation-induced colitis. No toxicity occurred.

MSC therapy was safe and effective on pain, diarrhea, haemorrhage, inflammation, fibrosis and limited fistulisation. For patients with refractory chronic inflammatory and fistulising bowel diseases, systemic MSC injections represent a safe option for salvage therapy. A clinical phase  II trial will start in 2018

Speaker
Biography:

Jaeseo Lee is a MS candidate at the Kyung Hee University, and she began researching in the Kwon lab in Jan of 2016. She has her expertise in tissue engineering and biomaterials. She is working to research drug delivery system using graphene quantum dots. She is also leading a project to develop drug delivery vehicles to treat specific breast cancer.

Abstract:

Graphene Quantum Dots (GQD) have been widely used for theragnosis for cancer disease. However, one of the current challenges is considerably difficult to release therapeutic agent to target site. In this study, we modified GQD that introduced herceptin through polyethylene glycol (PEG) and disulfide bond to induce active targeting for specific cells and control the drug release. The resulting of UV spectroscopy, Fluorescence, Fourier Transform Infrared showed that GQD-complex (GQD-comp) was conjugated successfully. The cell viability was presented that GQD-comp had a substantial effect on HER2-overexpressing breast cancer cells. In endosomal condition of low pH and high glutathione, doxorubicin was rapidly released from Doxorubicin loaded GQD-comp. Besides, this optical imaging by confocal laser scanning microscopy indicated to certainly confirm intracellular trafficking. In conclusion, GQD-comp had potential theragnostic agents to HER2-positive breast cancer cells. 

Speaker
Biography:

Donghyun Lee is a Ph.D. candidate at the Kyung Hee University, and he began researching in the Kwon lab in May of 2013. He has his expertise in tissue engineering and biomaterials. He is working to research biological properties of gelatin hydrogel and gold nanoparticle with the goal of designing injectable material systems for bone regeneration. He is also leading a project to develop biomaterial to treat alveolar bone defects. He has published 4 scientific research papers as a first author and 18 papers as a co-author.

Abstract:

Poly(lactide-co-glycolide) (PLGA) is a biocompatible and biodegradable polymer that has been widely used in devices for tissue engineering and drug delivery applications. Gold nanoparticles (GNPs) have also been used as biomaterials and have been found to have a positive effect on bone formation. In this study, we synthesized thiol end-capped PLGA (PLGA-SH) and used it for binding GNPs. This PLGA was processed into a sheet form via electrospinning. GNPs with an approximate size of 30 nm were attached onto the PLGA-SH sheet surfaces (PLGA-GNPs). This membrane was characterized by thermogravimetric analysis, ultraviolet/visible spectrophotometry, field emission scanning electron microscopy, energy dispersive X-ray spectroscopy, X-ray photoelectron spectroscopy, and confocal laser scanning microscopy. Characterization results show that the GNPs are well attached on the PLGA-SH sheet and it is possible to control the GNPs load. Additionally, in-vitro results showed that PLGA-GNPs have good biocompatibility. They were also found to enhance osteogenic differentiation of human adipose derived stem cells. From these results, we have determined that the PLGA-GNP fibers can be useful as materials for bone regeneration and can also potentially serve as drug carriers.

Speaker
Biography:

Abstract:

The purpose of study is to explore the efficacy and safety of platelet rich plasma (PRP) in the non-operative management of shoulder tendinopathy amongst individuals with spinal cord injury.

In this case in road traffic accidents that cause spinal injuries to the central nervous system, which increased morbidity and motility. The complications in patients were developed, resulting to a challenging problem for medicine. Platelet is naturally involved in haemostasis and tissue healing processes due to their content in growth factor and other bioactive molecules. Basic science and preclinical evidence supports the use of platelet derived growth factors as well as of PRP for enhancing reparatory processes in spinal cord injury.

In recent advancement in the field of regenerative medicine it was thought that this would grant a new approach to this problem, as it had proven beneficial in the repair of peripheral nerve injuries and their regeneration. This study was done to evaluate the effect of Platelet Rich Plasma (PRP) and its effect on the spinal cord post trauma. Subsequently, it was seen that PRP proved beneficial with marked positive effects in both muscle tone and muscle control and marked clinical improvement, although it can still be said that further research must be done in this field.

Clinical results about the use of PRP for bone, tendon, cartilage or spinal cord healing are encouraging and continue to accumulate in the recent years. Proteomic profiling and biomarker based PRP characterization have the potential of advancing the field of PRP application. High quality studies are awaited in order to enable clear cut therapeutic indications.

Speaker
Biography:

Marketa Benkova, née Pasdiorova, obtained her Master degree of Medical bioanalytics at the Faculty of Pharmacy in Hradec Kralove, Charles University in Prague in 2014. She worked for one year as a laboratory technician at the Department of Clinical Microbiology, University Hospital in Hradec Kralove. Currently, she is the postgraduate student of the Faculty of Military Health Sciences, University of Defence, and she also works as a researcher at Biomedical Research Center, University Hospital in Hradec Kralove. She is author or co-author of 15 publications in reputed journals.       

Abstract:

Three series of N-alkylammonium salts (7a-c, 8a-b, 9a-b) based on quaternary ammonium compounds with a different length and type of carbon chain (C12, C14, C16) were synthesized, characterized (EA, HRMS, NMR) and tested in vitro for antimicrobial (antibacterial, antifungal and antialgal) activity. Furthermore, the critical micelle concentration (CMC), capacity factors k and cytotoxicity were likewise measured to elucidate possible structure-activity relationships. The antimicrobial activity of the prepared compounds has been evaluated and compared. All compounds being tested proved high efficacy against both Gram-positive and Gram-negative bacterial strains, excluding the activity against multi-resistant Pseudomonas aeruginosa. Antifungal testing showed high activity of most compounds against fungal strains (yeasts and filamentous fungi) except of Aspergillus niger. The relationship between length of carbon chain and the efficiency has been observed. Series 7a-c proved high antialgal efficacy. Cell viability assay confirmed an expected trend that increasing carbon chain length results in higher cytotoxicity.

 

Speaker
Biography:

Staphylococci are notorious human pathogens that cause variety of diseases ranging from minor skin infections to chronic systemic infections such as osteomyelitis, endocarditis and biofilm associated infections of in dwelling devices. These infections have also the potential to spread both in health care facilities and communities eventually causing minor outbreaks. A multitude of factors including but not limited to antibiotic resistance, biofilm production and formation of antibiotic tolerant persister cells contribute to treatment failures in such staphylococcal infections.

Abstract:

A total of 375 Staphylococci were isolated from clinical samples received in a tertiary care hospital of Nepal during 2015-2017 were included to study antibiotic susceptibility pattern, biofilm formation and persister cells development. Standard microbiological procedure was used to identify staphylococcal species and CLSI guideline was followed to determine antibiotic susceptibility pattern. Biofilm production was detected using both phenotypic and genotypic methods. Among 375 Staphylococcal isolates, 161 isolates were identified as S. aureus comprising 81% methicillin resistant
and 19% methicillin sensitive S. aureus and remaining coagulase negative Staphylococcus spp. While most of the isolates were resistant to Penicillin, these were sensitive towards Chloramphenicol, Tetracycline and Clindamycin. Among different phenotypic assays used for detection of biofilm formation, tissue culture method detected biofilm production in 22.1% isolates followed by tube method and Congo red agar method which detected only 16.8% and 5.3%, respectively. On further verification, PCR detected ica genes in 23% of staphylococcal isolates thus revealing the biofilm formation in these samples.
In-vitro persister assay is undergoing to study the causal link between persister cells and treatment failures in these isolates.

Speaker
Biography:

Dr. El Hadji Seydou Mbaye was born in 1978 in Kaolack a region of Senegal. During 2008-2013, he persued his PhD in Biology and Human Pathologies with the collaboration of the International Agency for Research on Cancer (IARC) /WHO, Lyon (France); 2006-2007 : Master of Life and Health, Specialty Biology of microorganisms, Virology in Louis Pasteur University of Strasbourg (France); 2005-2006 : Master of Life and Health, option of Immunophysiopathology in Louis Pasteur University of Strasbourg (France); 2004-2005 : License of Biochemistry in Louis Pasteur University of Strasbourg (France); 2002-2004 : General Degree in Sciences and Technologies in University of METZ (France). He was certified by the Federation International of Gynecology Obstetrics (FIGO), the Accreditation Council of Oncology in Europe (ACOE, www.acoe.be), the Institute Catalan of Oncology (ICO) for cervical cancer prevention (Grade 10/10) in support of Continuing Medical Education for physicians. These credits are also recognised as Physician’s Recognition Award (AMA PRA Category 1 credits) by the American Medical Association. He was certified, by the United Nations for Basic Notion of Security on the Ground-Protection, Health and behavior, by the International Agency for Research on Cancer (IARC)/World Health Organization, Lyon (France) for Safety Certificate. He has published 1 Book with a style of philosophical story. Author of the world program against cancer in low and middle incomes countries, he is lead author (first listed) of more than 90 peer-reviewed articles published in reputed journals. He is Editorial Board Member of the International Journal of Immunology, associate membership of the World Society for Virology and also, member of BCNet International Working Group, International Agency for Research on Cancer (IARC)/World Health Organization (WHO).

 

Abstract:

Cervical cancer is the most frequent cancer among women in Senegal. However, there are few data concerning the HPV types inducing neoplasia and cervical cancers and their prevalence, in the general population of Senegal The aim of this study is to determine the prevalence of HPV infection in Senegalese women aged from 18 years and olderA study was performed on 498 cervix samples collected from healthy women aged 18 and older in Dakar. 438 other samples were collected from three other regions, Thiès, Saint Louis and Louga. The samples were screened for 21 HPV genotypes using an HPV type-specific E7 PCR bead-based multiplex genotyping assay (TS-MPG) which is a laboratory-developed method for the detection of HPVThe prevalence for pHR/HR-HPV in the region of Dakar was 20.68%. HPV 52 (3.21%) was the most prevalent HPV type, followed by HPV 16 (3.01%) and HPV 31 (3.01%). In the regions of Thiès, Louga and Saint Louis, the prevalence for pHR/HR-HPV was 29.19%, 23.15% and 20%, respectively

 

 

Speaker
Biography:

Research areas mainly   infectious diseases like Leprosy, and Tuberculosis I am working in this Institute since last more than 30 years I have done MSc., M.Phil & Ph.D from Agra College, throughout Ist class I am  guiding Ph.D,students Project and Training students who wish to do research work in biomedical fields.I  got  an  opportunity  to take  my  basic training by eminent electron microscopistProf. M. Nishiura, from Kyoto University, Japan for more than 2 years. I also privileged to take the training on “Cell Ultra structural   and Electron Microscopy” under Prof. B.K.Ghosh, Ruttgers University, New Jersey, U.S.A. in WHO workshop.

 

Abstract:

Electron microscopy used to investigate the detailed structure and configuration of the mycobacteria which may lead to an understanding the role of  infections  and in transmitting the diseases. In the natural world more than 99% of bacteria exist as biofilms and according to NIH report more then 68% of all human infections are associated with  biofilms formation. Biofilms are slimy, glue-like substance that excreted by bacteria and aggregate on living surface. Biofilms are formed to protect the bacteria from host defences, antibiotics and from harsh environmental conditions. Biofilms are found almost everywhere in nature, including soil, water pipes, and even inside the human body. Many Mycobacteria  can develop biofilm, a multicellular structure largely combining bacteria and their extracellular polymeric substances (EPS). The formation of biofilm results in an alternative existence in which microbes ensure their survival in adverse environments. Bacterial biofilms are often associated with  infections specially with medical implants such as catheters and other medical devices. Attachment of mycobacteria involved in biofilm formation in the liquid air interface is a complex process, with many variables such as pH, nutrient levels, iron, oxygen, ionic strength and temperature, affecting the outcome.

We had taken two mycobacterial species for study of Mycobacterial biofilm. The isolates were subcultured and characterized biochemically and molecularly. M.avium developed strong amount of biofilm at 30oC and 42oC  as compared to 370C. M tuberculosis (H37Rv) developed strong biofilm at 370C  and no biofilm at 300C and 420C in MB 7H9 media and Sauton’s media. The selected non tuberculosis mycobacteria  and  H37Rv developed strong biofilm in the presence of OADC enrichment in MB7H9   as well as Sauton’s medium. Isoniazid showed strong inhibited  biofilm in fast grower and sensitive isolates. However, Pyrazinamide and Isoniazid  inhibited  biofilm of M.tuberculosis (H37Rv) and in MDR isolates Ethionamide and Moxifloxacin inhibited biofilm in slow grower and fast grower Mycobacteria. However, many  mycobacterial species are known to form biofilms, little is known about either  the genetic requirements, patterns of gene expression. In micro array hybridisation we have found that six genes were  expressed in M.avium.  In M. tuberculosis MDR isolates seven genes were expressed and two genes Rv0359 and Rv3526 were homologus as earlier reported in  P. areuginosa and M. avium  which might be responsible for biofilm formation.

 

Speaker
Biography:

I Fatoba Johnson Sunday was born on the 19th of May, 1991. I hail from Aisegba Ekiti Ekiti State Nigeria where I was also born and bred. Being the third of a family of five, it was a privilege that refined my character in teaching me humility and responsibility, and also developing my leadership acumen as I saw my younger ones grow up. I hold a bachelor's degree in Microbiology from Ekiti State university and also Master's degree program in Medical Microbiology (Bacteriology) at the University of Benin, Edo State. Sequel to my passion for academics, I have my passion geared towards reading journals, textbooks, research, periodicals. And this has widened my knowledge in the academic field and has also narrowed my focus in medical microbiology. Towards career development, I currently lecture Communicable disease and immunology at the Grand Royal College of Health Technology, Agbado Ekiti. And my wealth of experience and exposure enables me to present education in its simplest form as I impart knowledge and character to the pupils. Owing to these career trend. I intend to vehemently pursue my academic goals with all diligence as I make contribution to knowledge in my country Nigeria and beyond.

Abstract:

Enterococcus faecalis is Gram-positive, catalase-negative, non-spore-forming, facultative anaerobic bacteria, which usually inhabit the alimentary tract of humans in addition to being isolated from environmental and animal sources. They are able to survive hostile environments, such as in extreme temperature (5-650C), pH (4.5-10.0) and high NaCl concentration, enabling them to colonize a wide range of niches. Depending on the site of infection this organism colonizes, invades the host tissue using their surface adhensin causing pathological changes in the host.  Enterococcus faecalis is the leading causative agent of serious nosocomial infections such as urinary tract infection (UTIs), endocarditis, bacteremia, intra-abdominal and intra-pelvic abscesses. This organism may frequently be acquired by cross-infection from other patients and through contaminated medical equipments. Diseases cause by this organism can be treated with antibiotics such as gentamycin, vancomycin and daptomycin. Cautious use of antibiotics and implementation of infection control measures are ways of preventing this infection. Understanding the pathogenicity and antibiotics resistance of this organism will help in preventing it from causing nosocomial infection

Speaker
Biography:

Abstract:

To explore the case management and disease burden of bacterial meningitis among children, admitted to Benghazi hospital from (January 2014 - December 2015), to study the trends of bacterial aetiology amongst cases of meningitis. and to isolate, identify and determine the antibiotic susceptibility patterns of pathogens associated with bacterial meningitis Cerebrospinal fluid (CSF) samples were collected from children admitted to children hospital units aged from few days up to 13 years old, and suspected to have bacterial meningitis, Doctor will almost always do a lumbar puncture.  In this retrospective study, the data were collected from the laboratory records and statistical unit. A total of 6306 CSF children samples from clinically suspected case of meningitis were subjected to microbiologic unit. There were (400) cases clinically diagnostic as BM in children, 103 cases were found to be CSF bacterial culture positive and (77) were positive by gram stain (41%). Most of meningitis cases were found in male’s children 225 (56%) than females175 (44%). The ratio of male to female was (1.3-1). Fever was the most common presentation (90%) followed by vomiting (60%), poor oral intake (51%). The mortality rate was (7%), Coagulase negative staphylococcus was the most frequent pathogens isolated (55%)followed by E. coli (15.5%)then staph aureus (14.6), followed by streptococcus pneumonia (11.7%), Klebsiella pneumoniae (9.7%), Acinetobacter species, (5.8%), then Pseudomonas species, (4.9%), and among the seasonal effect the highest incidence in the study area were occurred on Augustus.