Scientific Program

Conference Series Ltd invites all the participants across the globe to attend 12th Annual Meet on Bacteriology & Applied Microbiology Singapore.

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Scientific Program Day 1
Scientific Program Day 2
Scientific Program Day 3

Day 1 :

Keynote Forum

Dr Donald Kuah

Sydney Sportsmed Specialists, Australia

Keynote: A phase 1 double-blind randomized controlled trial of allogeneic adipose derived mesenchymal stem cells (Progenza) for treatment of osteoarthritic knees

Time : 10:00-10:40

Conference Series Bacteriology Asia Pacific 2018 International Conference Keynote Speaker Dr Donald Kuah photo
Biography:

Donald Kuah is an experienced Sports and Exercise Physician specialist and one of the founding practitioners at Sydney Sports Medicine Centre in Olympic Park. He has been the Chief Medical Officer to the Wests Tigers NRL team (2003-2013), is the current Chairman of the NRL Medical Advisory Panel and is on the Olympic Winter Institute of Australia medical panel. He was the NSW Institute of Sport Medical Director from 2000-2012, being responsible for the state’s top 700 elite athletes and remains involved with treating NSWIS athletes. Dr Kuah is involved in teaching at many levels and also heavily involved in clinical research. He
has previously been the State Training Coordinator for the Australasian College of Sport and Exercise Medicine and continues to be a Clinical Training Supervisorand an Examiner for the College. His interest areas lie in treatment of tendon pathologies, osteoarthritis, hamstring origin pathologies and also in the area of
regenerative medicine.

Abstract:

Osteoarthritis (OA) is a debilitating condition with no known cure or disease-modifying treatments available. Stem cell therapies may have the potential to halt OA disease progression and repair and regenerate affected joints. Bone Marrow (BM) and adipose-derived (AD) MSCs are being trialed for multiple diseases including OA but the majority of research has involved use of autologous cells (rather than allogeneic). A brief review of the literature will be presented. Two studies of knee OA patients receiving allogeneic BM-MSCs concluded MSCs were safe but varying efficacy results were reported. One study reported significant decreases in pain and encouraging MRI results in the MSC treated group. The second study observed no significant changes in objective and subjective pain measurements and no cartilage improvements by MRI. Allogeneic MSC safety has also been demonstrated in a cartilage defect study and in a study delivering the MSCs post partial meniscectomy. These studies reported improvements in pain and MRI measurements in patients that received MSCs. The STEP (Safety, tolerability and Efficacy of Progenza) Trial was a first-in-human, Phase I, randomised, double-blind, placebo-controlled single
ascending dose study of Progenza (PRG) for OA. PRG is composed of in vitro expanded AD-MSCs derived from human donor
adipose tissue combined with cell culture supernatant, frozen in ready to inject vials. The primary objective was to evaluate the
safety and tolerability of PRG administered via a single intra-articular injection. Secondary objectives included investigating the
effect of PRG on pain, function and joint structures in the study knee, quality of life and relevant serum and urine biomarkers.
Twenty patients (40-65 years; Kellgren-Lawrence grade 1-3 knee OA and 35-90mm visual analogue scale pain score) were
treated in two cohorts of 10 patients with a 12 month follow-up. Cohort 1: 3.9 million cells (n=8) or placebo (n=2) and cohort
2: 6.7 million cells (n=8) or placebo (n=2). All patients experienced at least 1 treatment emergent adverse event. Most were
mild and unrelated to the Investigational Product (IP). No serious adverse events were reported, and no patients withdrew
from the study due to an AE. VAS pain scores (Fig. 1) and WOMAC pain subscale scores significantly improved from baseline
at all timepoints in the PRG groups (p < 0.05) whilst only a marginal non-statistically significant improvement was observed
in the placebo group. Lateral tibial cartilage volume was preserved in the PRG 3.9M group whilst the placebo group showed a
statistically significant cartilage loss. PRG was safe and well tolerated when administered as a single intra-articular injection to
patients with symptomatic knee OA. Improvements in knee structure outcomes demonstrates the potential for PRG to have a
disease modifying effect in OA joints which warrants further investigation.

Keynote Forum

Christoper John N Tibayan

Ospital Ng Makati, Philippines

Keynote: Second day sequential organ failure assessment score as predictor in 30-day hospital mortality among Filipino adult patients who presented with sepsis at the emergency department: A prospective Cohort study

Time : 10:40-11:20

Conference Series Bacteriology Asia Pacific 2018 International Conference Keynote Speaker Christoper John N Tibayan photo
Biography:

Christoper John N Tibayan is a Diplomat in Internal Medicine and is a Member of the Philippine College of Physicians. He has completed his Doctorate in Medicine from Far Eastern University.

Abstract:

Sepsis-3 Guidelines promoted the new definition and prognostication scheme among patients with sepsis using the Sequential Organ Failure Assessment (SOFA) Scoring System. The study determined the discriminative power of the 2nd day SOFA scoring system in predicting 30-day hospital mortality among adult Filipino patients who presented with sepsis and suggested adjustment on the applicability of SOFA scoring system in a resource limited government hospital in the Philippines. We evaluated 107 adults with sepsis presenting at the emergency department from June to August 2017 at Ospital Ng
Makati, Philippines. Receiving operating characteristic curves were generated to determine optimal cut off scores of SOFA in
predicting 30-day mortality. Binary logistic regression was performed to determine the association of the SOFA derivatives with
hospital mortality.

Keynote Forum

Eman Khalifa

Alexandria University, Egypt

Keynote: Biodiversity of TOXIGENIC FUNGI in livestock and poultry feedstuffs in Egypt

Time : 09:50

Conference Series Bacteriology Asia Pacific 2018 International Conference Keynote Speaker Eman Khalifa photo
Biography:

Acting Dean of Faculty of Veterinary Medicine, Alexandria University, Matrouh Branch.

 

Abstract:

This study was aimed to investigate the prevalence toxigenic fungi and mycotoxines, a total no. of 90 samples representing various types of animal and poultry feedstuffs were collected from different localities in Egypt; Alexandria, Beheira, New Valley, Assiut and Cairo, The results showed that 78 with a percentage of 86.7% of samples were contaminated with different fungal species, the fungal load was ranged from 0.4 to 12 x 10³ cfu/gram among different samples,  Aspergillus was the most common genus being isolated from all mycologically positive samples with total count of 101900 colonies per gram matching 83.84% of total fungal population. 16 species of Aspergillus; which A. flavus and A. niger were the most common colonizing 72.2% and 56.6% of samples matching 15.8% and 41.7% of total fungal count. Penicillium appeared in 50% of samples accounting for 13.1% of total fungal population. Also in the present study 13 feedstuff samples were analyzed for mycotoxin contamination. These samples were mycologically positive yielding low (40-600 cfu/g) or high number of fungal colonies (1280-4920 cfu/g). A. flavus and A. niger were the dominant species in these samples, aflatoxin B1  at level of 0.851 and 1.363 ug/kg were detected in 2 samples; respectively. Aflatoxin B2 at a level of 0.479 ug/kg was also detected in 1 sample. Levels of Aflatoxins in the positive samples (AFB1 and AFB2) are below the recommended limit for animal feedstuff ingredients for beef cattle (20 ug/kg). From previously mentioned data, it was clear that feedstaffs of animal and poultry in Egypt represented hazardous source of toxigenic fungi which harm animal and poultry health, decrease their production, and could be serious sources of human illness, so regular screening in animal and poultry feedstaffs in Egypt is a good practice in order to control and overcome their presence and dangers. 

 

Keynote Forum

Gomathi Swaminathan

J.K.K.Nattraja College of Pharmacy, India

Keynote: Phytochemical Analysis and Neuroprotective activity of PEDALIUM MUREX LINN Leaves against Bacterial Cell Wall Content LIPOPOLYSACCHARIDE-Induced Endotoxemia

Time : 11:40-12:20

Conference Series Bacteriology Asia Pacific 2018 International Conference Keynote Speaker Gomathi Swaminathan photo
Biography:

Mrs. Gomathi Swaminathan, M.Pharm., is perusing part-time Ph.D in The Tamil Nadu Dr. M.G.R Medical University, Chennai, Tamilnadu, India and is a full-time faculty of J. K. K. Nattraja College of Pharmacy, Kumarapalayam, Tamilnadu, India. Her field of doctoral and research work is primarily on herbal phytoconstituents and medicines for neurodegenerative disorders. She has published 20 research articles in various national and international journals and has received many best poster and oral presentation awards, young scientist award in various national and international conferences.     

Abstract:

Neurodegenerative diseases (ND) are threatening worldwide. Huge number of behavioural changes are associated with ND such as depression, anxiety, memory loss, stress and so on. Effective drugs need to cure the ND from nature. In this present study, we had evaluated the phytochemical analysis and neuroprotective activity of Pedalium murex Linn (PM) leaves against bacterial cell wall content Lipopolysaccharide (LPS)-induced endotoxemia in rats. Two compounds were isolated from 90% v/v of ethanol extract of Pedalium murex Linn (EEPM) named as PM I, PM II. Spectral data of isolated compounds strongly suggested that PM I showed the structural similarities with 4´, 5, 7- trihydroxy flavone may be Apigenin and PM II showed the structural similarities with 3,5,7-Trihydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one which may have the presence of kaempferol. The data of our present study revealed that LPS significantly decreases the food, water intake and body mass which was recovered EEPM treated rats.  EEPM attenuated or completely abolished the symptoms of depression and anxiety. Antioxidant enzymes includes SOD, CAT, GR, GPX levels were decreased in LPS-treated rats, which was recovered by EEPM treated rats. Histopathological results also support the neuroprotective effect of EEPM against LPS-induced neurodegeneration. We  may conclude that EEPM may be a potential candidate for LPS-induced brain damage which may be attributed to the presence of potent antioxidants in EEPM

  • Bacterial Diseases | Bacterial Epidemiology | Bacterial Pathogenesis | Plant Bacteriology | Bacterial Morphology & Metabolism | Bacterial Identification Method

Session Introduction

Donald Kuah

Sydney Sportsmed Specialists, Australia

Title: Second Day Sequential Organ Failure Assessment (SOFA) Score as Predictor in 30–Day Hospital Mortality among Filipino Adult Patients who presented with Sepsis at the Emergency Department
Biography:

Donald Kuah is an experienced Sports and Exercise Physician specialist and one of the founding practitioners at Sydney Sports Medicine Centre in Olympic Park. He has been the Chief Medical Officer to the Wests Tigers NRL team (2003-2013), is the current Chairman of the NRL Medical Advisory Panel and is on the Olympic Winter Institute of Australia medical panel. He was the NSW Institute of Sport Medical Director from 2000-2012, being responsible for the state’s top 700 elite athletes and remains involved with treating NSWIS athletes. Dr Kuah is involved in teaching at many levels and also heavily involved in clinical research. He
has previously been the State Training Coordinator for the Australasian College of Sport and Exercise Medicine and continues to be a Clinical Training Supervisorand an Examiner for the College. His interest areas lie in treatment of tendon pathologies, osteoarthritis, hamstring origin pathologies and also in the area of
regenerative medicine.

Abstract:

Osteoarthritis (OA) is a debilitating condition with no known cure or disease-modifying treatments available. Stem cell therapies may have the potential to halt OA disease progression and repair and regenerate affected joints. Bone Marrow (BM) and adipose-derived (AD) MSCs are being trialed for multiple diseases including OA but the majority of research has involved use of autologous cells (rather than allogeneic). A brief review of the literature will be presented. Two studies of knee OA patients receiving allogeneic BM-MSCs concluded MSCs were safe but varying efficacy results were reported. One study reported significant decreases in pain and encouraging MRI results in the MSC treated group. The second study observed no significant changes in objective and subjective pain measurements and no cartilage improvements by MRI. Allogeneic MSC safety has also been demonstrated in a cartilage defect study and in a study delivering the MSCs post partial meniscectomy. These studies reported improvements in pain and MRI measurements in patients that received MSCs. The STEP (Safety, tolerability and Efficacy of Progenza) Trial was a first-in-human, Phase I, randomised, double-blind, placebo-controlled single
ascending dose study of Progenza (PRG) for OA. PRG is composed of in vitro expanded AD-MSCs derived from human donor
adipose tissue combined with cell culture supernatant, frozen in ready to inject vials. The primary objective was to evaluate the
safety and tolerability of PRG administered via a single intra-articular injection. Secondary objectives included investigating the
effect of PRG on pain, function and joint structures in the study knee, quality of life and relevant serum and urine biomarkers.
Twenty patients (40-65 years; Kellgren-Lawrence grade 1-3 knee OA and 35-90mm visual analogue scale pain score) were
treated in two cohorts of 10 patients with a 12 month follow-up. Cohort 1: 3.9 million cells (n=8) or placebo (n=2) and cohort
2: 6.7 million cells (n=8) or placebo (n=2). All patients experienced at least 1 treatment emergent adverse event. Most were
mild and unrelated to the Investigational Product (IP). No serious adverse events were reported, and no patients withdrew
from the study due to an AE. VAS pain scores (Fig. 1) and WOMAC pain subscale scores significantly improved from baseline
at all timepoints in the PRG groups (p < 0.05) whilst only a marginal non-statistically significant improvement was observed
in the placebo group. Lateral tibial cartilage volume was preserved in the PRG 3.9M group whilst the placebo group showed a
statistically significant cartilage loss. PRG was safe and well tolerated when administered as a single intra-articular injection to
patients with symptomatic knee OA. Improvements in knee structure outcomes demonstrates the potential for PRG to have a
disease modifying effect in OA joints which warrants further investigation.

Gomathi Swaminathan

JKKN College of Pharmacy, India

Title: Phytochemical Analysis and neuroprotective activity of Pedalium murex linn Leaves against bacterial cell wall Content lipopolysacchride-induced endotoxemia
Biography:

Mrs. Gomathi Swaminathan, M.Pharm., is perusing part-time Ph.D in The Tamil Nadu Dr. M.G.R Medical University, Chennai, Tamilnadu, India and is a full-time faculty of J. K. K. Nattraja College of Pharmacy, Kumarapalayam, Tamilnadu, India. Her field of doctoral and research work is primarily on herbal phytoconstituents and medicines for neurodegenerative disorders. She has published 20 research articles in various national and international journals and has received many best poster and oral presentation awards, young scientist award in various national and international conferences. 

Abstract:

Neurodegenerative diseases (ND) are threatening worldwide. Huge number of behavioural changes are associated with ND such as depression, anxiety, memory loss, stress and so on. Effective drugs need to cure the ND from nature. In this present study, we had evaluated the phytochemical analysis and neuroprotective activity of Pedalium murex Linn (PM) leaves against bacterial cell wall content Lipopolysaccharide (LPS)-induced endotoxemia in rats. Two compounds were isolated from 90% v/v of ethanol extract of Pedalium murex Linn (EEPM) named as PM I, PM II. Spectral data of isolated compounds strongly suggested that PM I showed the structural similarities with 4´, 5, 7- trihydroxy flavone may be Apigenin and PM II showed the structural similarities with 3,5,7-Trihydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one which may have the presence of kaempferol. The data of our present study revealed that LPS significantly decreases the food, water intake and body mass which was recovered EEPM treated rats.  EEPM attenuated or completely abolished the symptoms of depression and anxiety. Antioxidant enzymes includes SOD, CAT, GR, GPX levels were decreased in LPS-treated rats, which was recovered by EEPM treated rats. Histopathological results also support the neuroprotective effect of EEPM against LPS-induced neurodegeneration. We  may conclude that EEPM may be a potential candidate for LPS-induced brain damage which may be attributed to the presence of potent antioxidants in EEPM
 
 
 
 
 
 

Christoper John N Tibayan

Ospital Ng Makati, Philippines

Title: Second Day Sequential Organ Failure Assessment (SOFA) Score as Predictor in 30–Day Hospital Mortality among Filipino Adult Patients who presented with Sepsis at the Emergency Department
Biography:

Sepsis-3 Guidelines promoted the new definition and prognostication scheme among patients with sepsis using the Sequential Organ Failure Assessment (SOFA) Scoring System. The study determined the discriminative power of the 2nd day SOFA scoring system in predicting 30-day hospital mortality among adult Filipino patients who presented with sepsis and suggested adjustment on the applicability of SOFA scoring system in a resource limited government hospital in the Philippines. We evaluated 107 adults with sepsis presenting at the emergency department from June to August 2017 at Ospital Ng
Makati, Philippines. Receiving operating characteristic curves were generated to determine optimal cut off scores of SOFA in
predicting 30-day mortality. Binary logistic regression was performed to determine the association of the SOFA derivatives with
hospital mortality.   

Abstract:

Shivatra Talchai,

Medical Innovative Technology, Singapore

Title: Expansion of High Quality Mesenchymal Stem Cells On Xeno Free Cell Revtm Msc Exhq For Therapeutic Applications
Biography:

Zher Min Tan BS, Priyatharshini Murugan MS, Shivatra Talchai PhD, Veerapol Khemarangsan MD, SMIT Stem Cell – Singapore Medical Innovative Technology Pte Ltd, 50 Science Park Road, #04-03, The Kendall, Singapore 117406

 

Abstract:

Multipotent mesenchymal stem cells (MSCs) that have self-renewal properties and can differentiate into adipocytes, osteoblasts, chondrocytes and others. MSCs have been found to have immunomodulatory effects. Thus, MSCs represent an ideal source of stem cells for tissue engineering applications.

To ensure their quality, biocompatibility and consistency for clinical applications, it is essential for MSCs cultured ex vivo to retain of their self-renewal and differentiation capacities. Traditionally, fetal bovine serum (FBS), dubbed standard media (STD), is used as a supplement to support cells in in vitro culture. However, this is disadvantageous as FBS is posing clinical risks such as viral or prion contamination and immune rejection, as well as affecting the differentiation potential of MSCs. To produce high quality MSCs for clinical use, sera- and animal-free media are being developed. We therefore formulated a human platelet lysate-based, serum-free media, MSC ExHQ Cell RevTM media that can be used to expand MSCs while retaining their multipotent differentiation potentials. Here, we compare the properties of MSCs cultured in standard media (STD) versus in MSC ExHQ Cell RevTM media. We found that standard media leads to overexpression of MSC negative marker, CD19 while ExHQ media does not. Therefore, we suggest that MSC ExHQ Cell RevTM media is more appropriate media for high quality MSCs as well as for later clinical applications.

 

Fatoba Johnson Sunday

University of Benin, Nigeria

Title: Enterococcus Faecalis As Nosocomial Pathogen
Biography:

Abstract:

Eman Khalifa,

Alexandria University, Egypt

Title: Biodiversity of toxigenic fungi in livestock and poultry feedstuffs in Egypt
Speaker
Biography:

Eman Khalifa is a Faculty of Veterinary Medicine, Alexandria University, Matrouh Branch and Acting Dean of Faculty of Veterinary Medicine, Alexandria University, Matrouh Branch and Assistant Professor of Microbiology and Immunology and Manager of Information Technology (IT Unit) Expert in Biotechnology, Food Microbiology, animal Microbiology, Molecular diagnostic Microbiology and Had the first record in Egypt and worldwide for isolation and identification of Trichoderma pseudokoningii Rifai from Egyptian immunocompromised cattle with Mycobacterium bovis infection with international record from Mycological Center, Assiut
University, and published in Nature Precedings: hdl:10101/npre.2012.6928.1 : Posted 23 Feb 2012.

Abstract:

This study was aimed to investigate the prevalence toxigenic fungi and mycotoxins, a total no. of 90 samples representing various types of animal and poultry feedstuffs were collected from different localities in Egypt: Alexandria, Beheira, New Valley, Assiut and Cairo, The results showed that 78 (86.7%) of samples were contaminated with different fungal species, the fungal load was ranged from 0.4 to 12×10³ cfu/gram among different samples, Aspergillus was the most common genus being isolated from all mycological positive samples with total count of 101,900 colonies per gram matching 83.84% of total fungal
population. 16 species of Aspergillus which are A. flavus and A. niger were the most common colonizing 72.2% and 56.6% of samples matching 15.8% and 41.7% of total fungal count. Penicillium appeared in 50% of samples accounting for 13.1% of total fungal population. Also in the present study 13 feedstuff samples were analyzed for mycotoxin contamination. These samples
were mycological positive yielding low (40-600 cfu/g) or high number of fungal colonies (1280-4920 cfu/g). A. flavus and A. niger were the dominant species in these samples, aflatoxin B1 at level of 0.851 and 1.363 μg/kg were detected in 2 samples, respectively. Aflatoxin B2 at a level of 0.479 μg/kg was also detected in 1 sample. Levels of Aflatoxins in the positive samples
(AFB1 and AFB2) are below the recommended limit for animal feedstuff ingredients for beef cattle (20 μg/kg). From previously mentioned data, it was clear that feedstuffs of animal and poultry in Egypt represented hazardous source of toxigenic fungi which harm animal and poultry health, decrease their production, and could be serious sources of human illness, so regular
screening in animal and poultry feedstuffs in Egypt is a good practice in order to control and overcome their presence and
dangers.

Sarita Manandhar

Tribhuvan University, Nepal

Title: Study of antibiotic susceptibility pattern and biofilm formation among staphylococci isolated from tertiary care hospital
Speaker
Biography:

Staphylococci are notorious human pathogens that cause variety of diseases ranging from minor skin infections to chronic systemic infections such as osteomyelitis, endocarditis and biofilm associated infections of in dwelling devices. These infections have also the potential to spread both in health care facilities and communities eventually causing minor outbreaks. A multitude of factors including but not limited to antibiotic resistance, biofilm production and formation of antibiotic tolerant persister cells contribute to treatment failures in such staphylococcal infections.

Abstract:

A total of 375 Staphylococci were isolated from clinical samples received in a tertiary care hospital of Nepal during 2015-2017 were included to study antibiotic susceptibility pattern, biofilm formation and persister cells development. Standard microbiological procedure was used to identify staphylococcal species and CLSI guideline was followed to determine antibiotic susceptibility pattern. Biofilm production was detected using both phenotypic and genotypic methods. Among 375 Staphylococcal isolates, 161 isolates were identified as S. aureus comprising 81% methicillin resistant
and 19% methicillin sensitive S. aureus and remaining coagulase negative Staphylococcus spp. While most of the isolates were resistant to Penicillin, these were sensitive towards Chloramphenicol, Tetracycline and Clindamycin. Among different phenotypic assays used for detection of biofilm formation, tissue culture method detected biofilm production in 22.1% isolates followed by tube method and Congo red agar method which detected only 16.8% and 5.3%, respectively. On further verification, PCR detected ica genes in 23% of staphylococcal isolates thus revealing the biofilm formation in these samples.
In-vitro persister assay is undergoing to study the causal link between persister cells and treatment failures in these isolates

Pranab Roy

HaldiaInst of Technology, India

Title: Characterization of plant growth promoting bacteria isolated from nodule co-infecting a single leguminous plant
Speaker
Biography:

Pranab Roy has completed his Post-Doctoral Research from Yale University School of Medicine and University of Connecticut Health Centre, USA working onadvanced Molecular Biology. He has joined as Lecturer in Bose Institute where he characterized a novel repetitive DNA sequence by molecular cloning and
sequencing when these techniques were just being introduced in India in mid-80’s. He also joined industrial R&D to set up the Genetic Engineering Unit at
Hindustan Lever Research Centre, Mumbai. He is currently working at Haldia Institute of Technology, where his research interest was with plant growth promotingrhizobacteria from root nodules from Fenugreek (Methi), a leguminous plant

Abstract:

The green revolution ushered in a growth in the production of food crops in India. From a net importer of food grains, India became self-sufficient increasing the total production from 50 million metric tons in mid-sixties to 200 million metric tons in mid-eighties. This was possible due to higher yielding and hybrid varieties of seeds, increased input of chemical
fertilizers and insecticides or pesticides. However, the pitfalls of high usage of chemical fertilizers were deterioration in the quality of agricultural soil. The natural microbes present in the rhizospheric soil died due to excess chemicals and the depletion of organic carbon content. The water retention capacity was also adversely affected due to lower porosity of the soil, sometimes
the soil became acidic causing lower productivity of plants. To overcome all these problems, the use of organic manure and bio-fertilizers were introduced. These are beneficial to the crop plants due to the following properties like nitrogen fixation, inorganic phosphate solubilization, production of siderophore and production of ACC deaminase. Nitrogen fixation by symbiotic microorganisms like Rhizobium, occurring in the root nodules of leguminous plants have been reported long back. In our studies, aseptic root nodules of Fenugreek (Methi) were crushed to isolate a variety of nitrogen fixing microbes. Though microbiological and biochemical studies indicated these mucoid colonies all Gram negative with different morphologies to the
Rhizobium species, sequencing of 16s rRNA genes and molecular phylogeny showed three of the isolates (1) R1 Enterobactercloacae (KX687556) (2) R10 Pantoea dispersa (KX687557) and (3) R12 Enterobacter ludwigii (KX687554). The temperatureand salinity tolerance of these cultures were studied to increase these properties of abiotic stress tolerance. UV mutagenesiswas tried, varying the time of exposure of the plated cultures to UV radiation, the survivors (>1% of initial population) werescreened for growth at 0.5 M NaCl and at 44 °C. The selected mutants of R1, R10 and R12 were studied for the plant growthpromoting activities like IAA (growth hormone) production, siderophore production and phosphate solubilization. Finally,the effects of the isolated bacteria and mutants of each and a consortium of all three were pre-treated with methi seeds andgerminated in pots to find out their effects on the growth of the plants. These are being analyzed after 40 days of growth in the
soil.

Eman Khalifa

Alexandria University, Egypt

Title: Biodiversity of toxigenic fungi in livestock and poultry feedstuffs in Egypt

Time : 12:20-12:50

Speaker
Biography:

Eman Khalifa is a Faculty of Veterinary Medicine, Alexandria University, Matrouh Branch and Acting Dean of Faculty of Veterinary Medicine, Alexandria University,Matrouh Branch and Assistant Professor of Microbiology and Immunology and Manager of Information Technology (IT Unit) Expert in Biotechnology, Food Microbiology, animal Microbiology, Molecular diagnostic Microbiology and Had the first record in Egypt and worldwide for isolation and identification of Trichoderma pseudokoningii Rifai from Egyptian immunocompromised cattle with Mycobacterium bovis infection with international record from Mycological Center, Assiut University, and published in Nature Precedings: hdl:10101/npre.2012.6928.1 : Posted 23 Feb 2012.

 

Abstract:

This study was aimed to investigate the prevalence toxigenic fungi and mycotoxins, a total no. of 90 samples representing various types of animal and poultry feedstuffs were collected from different localities in Egypt: Alexandria, Beheira, New Valley, Assiut and Cairo, The results showed that 78 (86.7%) of samples were contaminated with different fungal species, the fungal load was ranged from 0.4 to 12×10³ cfu/gram among different samples, Aspergillus was the most common genus being isolated from all mycological positive samples with total count of 101,900 colonies per gram matching 83.84% of total fungal
population. 16 species of Aspergillus which are A. flavus and A. niger were the most common colonizing 72.2% and 56.6% of samples matching 15.8% and 41.7% of total fungal count. Penicillium appeared in 50% of samples accounting for 13.1% of total fungal population. Also in the present study 13 feedstuff samples were analyzed for mycotoxin contamination. These samples
were mycological positive yielding low (40-600 cfu/g) or high number of fungal colonies (1280-4920 cfu/g). A. flavus and A. niger were the dominant species in these samples, aflatoxin B1 at level of 0.851 and 1.363 μg/kg were detected in 2 samples, respectively. Aflatoxin B2 at a level of 0.479 μg/kg was also detected in 1 sample. Levels of Aflatoxins in the positive samples
(AFB1 and AFB2) are below the recommended limit for animal feedstuff ingredients for beef cattle (20 μg/kg). From previously mentioned data, it was clear that feedstuffs of animal and poultry in Egypt represented hazardous source of toxigenic fungi which harm animal and poultry health, decrease their production, and could be serious sources of human illness, so regular
screening in animal and poultry feedstuffs in Egypt is a good practice in order to control and overcome their presence and
dangers.

 

Sarita Manandhar

Tribhuvan University, Nepal

Title: Study of antibiotic susceptibility pattern and biofilm formation among staphylococci isolated from tertiary care hospital

Time : 13:40-14:10

Speaker
Biography:

Staphylococci are notorious human pathogens that cause variety of diseases ranging from minor skin infections to chronic systemic infections such as osteomyelitis, endocarditis and biofilm associated infections of in dwelling devices. These infections have also the potential to spread both in health care facilities and communities eventually causing minor outbreaks. A multitude of factors including but not limited to antibiotic resistance, biofilm production and formation of antibiotic tolerant persister cells contribute to treatment failures in such staphylococcal infections.

Abstract:

A total of 375 Staphylococci were isolated from clinical samples received in a tertiary care hospital of Nepal during 2015-2017 were included to study antibiotic susceptibility pattern, biofilm formation and persister cells development. Standard microbiological procedure was used to identify staphylococcal species and CLSI guideline was followed to determine antibiotic susceptibility pattern. Biofilm production was detected using both phenotypic and genotypic methods.Among 375 Staphylococcal isolates, 161 isolates were identified as S. aureus comprising 81% methicillin resistant and 19% methicillin sensitive S. aureus and remaining coagulase negative Staphylococcus spp. While most of the isolates were resistant to Penicillin, these were sensitive towards Chloramphenicol, Tetracycline and Clindamycin. Among different
phenotypic assays used for detection of biofilm formation, tissue culture method detected biofilm production in 22.1% isolates followed by tube method and Congo red agar method which detected only 16.8% and 5.3%, respectively. On further verification, PCR detected ica genes in 23% of staphylococcal isolates thus revealing the biofilm formation in these samples. In-vitro persister assay is undergoing to study the causal link between persister cells and treatment failures in these isolates.

Pranab Roy,

HaldiaInst of Technology, India

Title: Characterization of plant growth promoting bacteria isolated from nodule co-infecting a single leguminous plant

Time : 14:40-15:10

Speaker
Biography:

Pranab Roy has completed his Post-Doctoral Research from Yale University School of Medicine and University of Connecticut Health Centre, USA working onadvanced Molecular Biology. He has joined as Lecturer in Bose Institute where he characterized a novel repetitive DNA sequence by molecular cloning and
sequencing when these techniques were just being introduced in India in mid-80’s. He also joined industrial R&D to set up the Genetic Engineering Unit at
Hindustan Lever Research Centre, Mumbai. He is currently working at Haldia Institute of Technology, where his research interest was with plant growth promotingrhizobacteria from root nodules from Fenugreek (Methi), a leguminous plant.

Abstract:

The green revolution ushered in a growth in the production of food crops in India. From a net importer of food grains,India became self-sufficient increasing the total production from 50 million metric tons in mid-sixties to 200 million metric tons in mid-eighties. This was possible due to higher yielding and hybrid varieties of seeds, increased input of chemical
fertilizers and insecticides or pesticides. However, the pitfalls of high usage of chemical fertilizers were deterioration in the quality of agricultural soil. The natural microbes present in the rhizospheric soil died due to excess chemicals and the depletion of organic carbon content. The water retention capacity was also adversely affected due to lower porosity of the soil, sometimes
the soil became acidic causing lower productivity of plants. To overcome all these problems, the use of organic manure and bio-fertilizers were introduced. These are beneficial to the crop plants due to the following properties like nitrogen fixation, inorganic phosphate solubilization, production of siderophore and production of ACC deaminase. Nitrogen fixation by symbiotic microorganisms like Rhizobium, occurring in the root nodules of leguminous plants have been reported long back. In our studies, aseptic root nodules of Fenugreek (Methi) were crushed to isolate a variety of nitrogen fixing microbes. Though microbiological and biochemical studies indicated these mucoid colonies all Gram negative with different morphologies to the
Rhizobium species, sequencing of 16s rRNA genes and molecular phylogeny showed three of the isolates (1) R1 Enterobacter cloacae (KX687556) (2) R10 Pantoea dispersa (KX687557) and (3) R12 Enterobacter ludwigii (KX687554). The temperature and salinity tolerance of these cultures were studied to increase these properties of abiotic stress tolerance. UV mutagenesis
was tried, varying the time of exposure of the plated cultures to UV radiation, the survivors (>1% of initial population) were screened for growth at 0.5 M NaCl and at 44 °C. The selected mutants of R1, R10 and R12 were studied for the plant growth promoting activities like IAA (growth hormone) production, siderophore production and phosphate solubilization. Finally, the effects of the isolated bacteria and mutants of each and a consortium of all three were pre-treated with methi seeds and germinated in pots to find out their effects on the growth of the plants. These are being analyzed after 40 days of growth in the
soil.

Ilknur Tuncer,

Dokuz Eylul University, Turkey

Title: Phylogenetic diversity, antibiotic susceptibility and enzymatic activities of bacterial isolates from sediments of Eastern Mediterranean Sea

Time : 15:20-15:50

Speaker
Biography:

Ilknur Tuncer has received two BSc degrees in Chemistry and Genetics from Bogazici University, Turkey, MSc degree in Marine Sciences from Bangor University, UK and PhD degree in Marine Sciences from Dokuz Eylul University, Turkey. She has several books and publications. She is an Editorial Member for several scientific journals. Her current research interests are marine environment, microbiology, phylogeny, species-area relations, human health and medicine.

Abstract:

Phylogenetic diversity of isolated bacteria and biogeochemical variability of sediments in shallow and deep basins of Eastern Mediterranean Sea were studied. According to 16S rRNA gene sequence analysis, the strains isolated from the study area were belonged to the Firmicutes, Gammaproteobacteria and Actinobacteria. The present study provided highly diverse bacterial strains of 14 different families especially with probability of representing new taxa in addition to the dominant genus bacillus in each sediment samples. The actinomycetes were tested physiologically using commercial kits and found
that they utilized proteins rather than carbohydrates. Geochemical parameters of the sediments together with enzymatic activity results of the strains underlined the nitrogen limitation in the area. The highest antibiotic resistance for the Firmicutes and Gammaproteobacteria strains was obtained from coastal sediments compared to deep basins supported by a positive correlation between sediment size and antibiotic resistance. The influence of environmental parameters was observed as the variability of bacterial community composition such that phylogenetic diversity was much higher in higher taxa for shallower
regions, especially in North Aegean Sea as compared to deep basins of Eastern Mediterranean Sea as supported in hierarchical cluster analysis of the isolates and statistical analysis including principle component analysis.

Gulseren Aktas

Istanbul University, Turkey

Title: Activity of Vancomycin Combined with Linezolid and with Dalbavancin against Clinical VRE Strains

Time : 15:50-16:20

Speaker
Biography:

Abstract:

A total of 30 randomly-selected clinical VRE strains were included in the study. Each strain was isolated from different patients who were admitted to the different clinics of Istanbul University, Istanbul Faculty of Medicine’s Hospital. Bacterial identifications of the strains were undertaken using conventional routine methods. The antibiotics used in the study were
vancomycin, linezolid and dalbavancin. Resistance to agents tested was investigated by micro-broth dilution assay as described by the Clinical and Laboratory Standards Institute (CLSI). They were prepared in accordance with CLSI proposals. Cation Adjusted Mueller-Hinton Broth (CAMHB) was used for all experiments. Additionally, in the tests for dalbavancin, CAMHB
with 0.002% final concentration of polysorbate 80 was used. The inoculum of strain was adjusted to approximately 5×105 cfu/mL in the wells. The Minimum İnhibitory Concentration (MIC) was defined as the lowest concentration of antibiotic giving complete inhibition of visible growth and interpreted in accordance with CLSI guidelines. Quality control of the test procedures was monitored via testing of the reference strains S. aureus ATCC 29213 and E. faecalis ATCC 29212. In vitro activities of antibiotics in combination were assessed using the micro-broth checker board technique. The Fractional İnhibitory Concentration (FIC) indexes were calculated using the following formula: FICI=FICA+FICB. The FICI was interpreted as synergism, FICI≤0.5; additive/indifference, FICI δ0.5-≤4; antagonism, FICIδ4.

Sumon Ghosha

Chittagong Veterinary and Animal Sciences University, Bangladesh

Title: Trends and Clinico -epidemiological features of HUMAN RABIES cases in Bangladesh 2006-2017
Speaker
Biography:

Communicable Disease Control, Directorate General of Health Services, Ministry of Health and Family Welfare, Bangladesh
 

Abstract:

Background:

Bangladesh has reduced the death of rabies case-patients by 50% over the last few years. Clinical and epidemiological knowledge of human rabies is crucial to direct further research investigation and to implement disease control measures.

Methods: We used data from the rabies record book of the Infectious Disease Hospital (IDH), Dhaka, Bangladesh from 2006 to 2017. We reviewed the medical records of 422 patients reported from 2011 to 2015 who had a clinical diagnosis of rabies.

Results: We found a strong trend of declining human rabies cases in Bangladesh from 2006 to 2017 (p<0.001 and tau = -0.85). In Sen’s slope test we found, over the 12 year period the slope changes at the rate of 11.45 (95% CI: -14.00 to -8.00) annually (p<0.001). The declining trend of human rabies cases can be explained by the number of dogs being vaccinated against rabies during 2011 to 2017 (adjusted R2=0.55). Dogs with unknown vaccination status, comprised the majority of exposures (n=380, 90%) followed by cat (n=24, 6%), jackal (n=12, 3%) and mongoose (n=6, 1%). The majority (n=327, 78%) of the victims sought treatment from traditional healers and 12% (n=51) received post-exposure prophylaxis (PEP). There was a difference in incubation times between the sites of bites in which the patients were exposed. The shortest, longest and median reported incubation periods were 11, 1080 and 52 days respectively. Cases with a wound on the head and neck appeared to have a shorter incubation time than those with wounds at other sites. Clinical symptoms included hydrophobia (97%), acrophobia (84%), photophobia (10%), and hypersalivation (7%). The case-fatality rate was 100%.

 

Yeganeh Rezaee

Research Institute of Forests and Rangelands, Iran

Title: Applied and natural ways to controlling MICROBIOLOGY and BACTERIOLOGY growth in Iran
Speaker
Biography:

I am Iranian scientist. I did my PhD. and Research work on Photochemistry or chemistry (Ag). And up to now my personal life for doing research with our co-worker on cultivation, extraction, formulation and produce natural products out of medicinal and Aromatic plants. So we designed research apparatuses and pilot of essential oil distillation and herbal extraction. Published 6 Book in Persian language and international of two chapters of bio-activity on herbal extracts or essential oil. Finish lots of thesis with different topics on Msc. and PhD. with my students. Publish many papers on this topic in national and international (ISI) journals. Know my position are as scientist in Research Institute Forests and Rangelands (RIFR) -Tehran-IRAN, Chairman of "Union Medicinal plants of Iran" (UMPI) and chair member of "Iranian medicinal plants society" (IMPS). I got three national award abut selected and important medicinal plant as natural product or instead of synthetic medicine and bio-logical uses. And so on…

 

Abstract:

The increasing demand in finding novel natural compounds or product in the world has diverted scientist’s attention toward of this sources. The very diverse environmental conditions and the rich variety of natural resources made it possible for the Iranians to create a great and flourishing civilization. Iran has hi ranks in medicinal and aromatic plant in natural resource and cultivated manner, traditional knowledge and producing of natural products in the world spatially in Middle East. Iran also is very famous in producing essential oil and water extraction from national plants spatially Rosa damacena, menthe spp and Thym spp. The methods of extraction in Rosa damascena cultivated in extensive zone of Iran are shown different constitution. All Samples were analyzed by GC and GC/MS.  The main constituents of oil extracted traditionally, samples were extracted by two hydro distillation method which designed by authors in Research Institute of Forests and Rangelands. Main components of essential oils were citronellol, cis-p-menth -2-en-1-ol and geraniol. These compounds and others are very popular in producing drug anti-cancer, anti-bacterial out of that and we should take care of over doses or toxicology. From my research work, going to presenting the natural constituents and traditionally patients consuming herbal applied and natural ways to controlling different Bacteria growing , as every scientist now reach to the point most of microbes and fungi or…. Become resistant to chemicals uses. So in this way we selected plants and main compounds out of them and got it best results. So it is necessary for a protected herbals and product to be provided with information on its ingredients, indications, dosage, pharmacology, contraindications and possible side effects before a rational decision on its use.

 

Abdullah Al-Mamari

Edna Adan University, Somaliland

Title: Prevalence of Bacterial Cerebrospinal Meningitis in Infants Attending Federal Medical Center BIRNIN KEBBI And INVITRO ANTIBIOTIC Sensitivity Test
Speaker
Biography:

Studies of oral fungal have indicated that prevalence of Candida was significantly higher in diabetics both in healthy controls and complete denture wearers compared to non -diabetics. The aims of study was to determine the prevalence oral Candidiasis among both diabetics and non–diabetics patients and evaluate the contribution of risk factors with the prevalence and distribution of Candida species with oral Candidiasis.

 

Abstract:

The present investigation was carried out in the department of medical microbiology and clinical samples were collected through regular visits to three main hospitals Al-Noor, Al-Amean, Al-Thawra and diagnosis was in the ALFA medical laboratory in Ibb City during period from January into November 2016 .In the current study 70 oral swabs samples were collected from surfaces of the upper of oral cavity and denture of all patients and then placed into a test tube containing 5ml subouraud's dextrose broth. The positive sample was processed for Gram's staining, Germ tube test, Chlamydospores test, Carbohydrate utilization medium examination and Chromagar test was made for differentiation between Candida a species. The results in this study showed that prevalence of Candida oral infection in Ibb City was 36(52%) positive with oral candidiasis out of 70 samples studied while, 34(48%) of patients studied were negative with oral candidiasis. On the other hand, the results also showed the distribution of oral Candida infection among diabetes and non-diabetes patients was 29(41%) and 7(11%) respectively, this results concordant with many previous studies. Candida albicans was the most prevalent types of Candida a species in this study. The results in current investigation also showed a significantly relationship between crews users, diabetes patients, age and oral candidiasis.

 

Musa Galadima

Kebbi State University of Science and Technology, Nigeria

Title: Prevalence of Bacterial Cerebrospinal Meningitis in Infants Attending Federal Medical Center BIRNIN KEBBI And INVITRO ANTIBIOTIC Sensitivity Test
Speaker
Biography:

Abstract:

A microbiological study was carried out on infants with suspected cases of cerebrospinal meningitis at Federal Medical Center Birnin Kebbi between February and June, 2017. Sixty six specimens of Cerebrospinal fluid (CSF) were obtained and examined using both microscopic and cultural methods for bacterial pathogens. Results obtained showed that only six (9.09%) of the specimens examined were positive for bacterial pathogens. The bacteria observed were Streptococcus pneumonia, Heamophilus influenza, Neisseria meningitides and Escheria coli. Invitro antibiotic sensitivity test carried on these isolated bacteria indicates that Ceftriaxone, Zinnacef and Gentamycin were active against all the isolates while Ciprofloxacin was active against E. coli. The result showed high prevalence of bacterial meningitis in contrast to its low incidence reported by many workers. There is the need to mount viral screening of CSF in addition to bacterial screening.

 

Murtala Saadu

Kebbi State University of Science and Technology, Nigeria

Title: synergistic effect of alkaloid and saponin of synergistic effect of ALKALOID and SAPONIN of Vernonia amygdalina on Escherichia coli
Speaker
Biography:

Abstract:

The study was aimed at investigate quantitatively phytochemical constituents of the leaf extract of Vernonia amygdalina, and determine the Synergistic effect of Alkaloid and Saponin compound of the plant extract against E. coli. The antibacterial activities of the alkaloid, saponin and their synergistic effect were carried out at varying concentration of 50, 25, 12.5 and 6.25mg/ml, using agar well diffusion method. The result of this study indicated the, ethanolic extract shows higher anti-bacterial activity at 100mg/ml concentration (11.29mm) compared to aqueous extract (9.24mm) against the test bacteria. It also showed that the anti-bacterial activity of the alkaloid content of the Vernonia amygdalina at 100mg/ml (11.01mm) was better than that of saponin (9.32mm) while the synergistic effect of alkaloid and saponin at concentration 100mg/ml (15.41mm). Were better than the individual compounds the result indicates the possibility of using the alkaloid and saponin compounds synergistically in the treatment of disease associated with E.coli.