Day 1 :
Keynote Forum
Eman Khalifa
Alexandria University, Egypt
Keynote: Biodiversity of TOXIGENIC FUNGI in livestock and poultry feedstuffs in Egypt
Time : 09:50

Biography:
Acting Dean of Faculty of Veterinary Medicine, Alexandria University, Matrouh Branch.
Abstract:
This study was aimed to investigate the prevalence toxigenic fungi and mycotoxines, a total no. of 90 samples representing various types of animal and poultry feedstuffs were collected from different localities in Egypt; Alexandria, Beheira, New Valley, Assiut and Cairo, The results showed that 78 with a percentage of 86.7% of samples were contaminated with different fungal species, the fungal load was ranged from 0.4 to 12 x 10³ cfu/gram among different samples, Aspergillus was the most common genus being isolated from all mycologically positive samples with total count of 101900 colonies per gram matching 83.84% of total fungal population. 16 species of Aspergillus; which A. flavus and A. niger were the most common colonizing 72.2% and 56.6% of samples matching 15.8% and 41.7% of total fungal count. Penicillium appeared in 50% of samples accounting for 13.1% of total fungal population. Also in the present study 13 feedstuff samples were analyzed for mycotoxin contamination. These samples were mycologically positive yielding low (40-600 cfu/g) or high number of fungal colonies (1280-4920 cfu/g). A. flavus and A. niger were the dominant species in these samples, aflatoxin B1 at level of 0.851 and 1.363 ug/kg were detected in 2 samples; respectively. Aflatoxin B2 at a level of 0.479 ug/kg was also detected in 1 sample. Levels of Aflatoxins in the positive samples (AFB1 and AFB2) are below the recommended limit for animal feedstuff ingredients for beef cattle (20 ug/kg). From previously mentioned data, it was clear that feedstaffs of animal and poultry in Egypt represented hazardous source of toxigenic fungi which harm animal and poultry health, decrease their production, and could be serious sources of human illness, so regular screening in animal and poultry feedstaffs in Egypt is a good practice in order to control and overcome their presence and dangers.
Keynote Forum
Gomathi Swaminathan
J.K.K.Nattraja College of Pharmacy, India
Keynote: Phytochemical Analysis and Neuroprotective activity of PEDALIUM MUREX LINN Leaves against Bacterial Cell Wall Content LIPOPOLYSACCHARIDE-Induced Endotoxemia
Time : 11:40-12:20

Biography:
Mrs. Gomathi Swaminathan, M.Pharm., is perusing part-time Ph.D in The Tamil Nadu Dr. M.G.R Medical University, Chennai, Tamilnadu, India and is a full-time faculty of J. K. K. Nattraja College of Pharmacy, Kumarapalayam, Tamilnadu, India. Her field of doctoral and research work is primarily on herbal phytoconstituents and medicines for neurodegenerative disorders. She has published 20 research articles in various national and international journals and has received many best poster and oral presentation awards, young scientist award in various national and international conferences.
Abstract:
Neurodegenerative diseases (ND) are threatening worldwide. Huge number of behavioural changes are associated with ND such as depression, anxiety, memory loss, stress and so on. Effective drugs need to cure the ND from nature. In this present study, we had evaluated the phytochemical analysis and neuroprotective activity of Pedalium murex Linn (PM) leaves against bacterial cell wall content Lipopolysaccharide (LPS)-induced endotoxemia in rats. Two compounds were isolated from 90% v/v of ethanol extract of Pedalium murex Linn (EEPM) named as PM I, PM II. Spectral data of isolated compounds strongly suggested that PM I showed the structural similarities with 4´, 5, 7- trihydroxy flavone may be Apigenin and PM II showed the structural similarities with 3,5,7-Trihydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one which may have the presence of kaempferol. The data of our present study revealed that LPS significantly decreases the food, water intake and body mass which was recovered EEPM treated rats. EEPM attenuated or completely abolished the symptoms of depression and anxiety. Antioxidant enzymes includes SOD, CAT, GR, GPX levels were decreased in LPS-treated rats, which was recovered by EEPM treated rats. Histopathological results also support the neuroprotective effect of EEPM against LPS-induced neurodegeneration. We may conclude that EEPM may be a potential candidate for LPS-induced brain damage which may be attributed to the presence of potent antioxidants in EEPM
Keynote Forum
Dr Donald Kuah
Sydney Sportsmed Specialists, Australia
Keynote: A phase 1 double-blind randomized controlled trial of allogeneic adipose derived mesenchymal stem cells (Progenza) for treatment of osteoarthritic knees
Time : 10:00-10:40

Biography:
Abstract:
Keynote Forum
Christoper John N Tibayan
Ospital Ng Makati, Philippines
Keynote: Second day sequential organ failure assessment score as predictor in 30-day hospital mortality among Filipino adult patients who presented with sepsis at the emergency department: A prospective Cohort study
Time : 10:40-11:20

Biography:
Abstract:
- Bacterial Diseases | Bacterial Epidemiology | Bacterial Pathogenesis | Plant Bacteriology | Bacterial Morphology & Metabolism | Bacterial Identification Method
Session Introduction
Eman Khalifa,
Alexandria University, Egypt
Title: Biodiversity of toxigenic fungi in livestock and poultry feedstuffs in Egypt

Biography:
Abstract:
Fatoba Johnson Sunday
University of Benin, Nigeria
Title: Enterococcus Faecalis As Nosocomial Pathogen
Biography:
Abstract:
Shivatra Talchai,
Medical Innovative Technology, Singapore
Title: Expansion of High Quality Mesenchymal Stem Cells On Xeno Free Cell Revtm Msc Exhq For Therapeutic Applications
Biography:
Zher Min Tan BS, Priyatharshini Murugan MS, Shivatra Talchai PhD, Veerapol Khemarangsan MD, SMIT Stem Cell – Singapore Medical Innovative Technology Pte Ltd, 50 Science Park Road, #04-03, The Kendall, Singapore 117406
Abstract:
Multipotent mesenchymal stem cells (MSCs) that have self-renewal properties and can differentiate into adipocytes, osteoblasts, chondrocytes and others. MSCs have been found to have immunomodulatory effects. Thus, MSCs represent an ideal source of stem cells for tissue engineering applications.
To ensure their quality, biocompatibility and consistency for clinical applications, it is essential for MSCs cultured ex vivo to retain of their self-renewal and differentiation capacities. Traditionally, fetal bovine serum (FBS), dubbed standard media (STD), is used as a supplement to support cells in in vitro culture. However, this is disadvantageous as FBS is posing clinical risks such as viral or prion contamination and immune rejection, as well as affecting the differentiation potential of MSCs. To produce high quality MSCs for clinical use, sera- and animal-free media are being developed. We therefore formulated a human platelet lysate-based, serum-free media, MSC ExHQ Cell RevTM media that can be used to expand MSCs while retaining their multipotent differentiation potentials. Here, we compare the properties of MSCs cultured in standard media (STD) versus in MSC ExHQ Cell RevTM media. We found that standard media leads to overexpression of MSC negative marker, CD19 while ExHQ media does not. Therefore, we suggest that MSC ExHQ Cell RevTM media is more appropriate media for high quality MSCs as well as for later clinical applications.
Biography:
Abstract:
Biography:
Mrs. Gomathi Swaminathan, M.Pharm., is perusing part-time Ph.D in The Tamil Nadu Dr. M.G.R Medical University, Chennai, Tamilnadu, India and is a full-time faculty of J. K. K. Nattraja College of Pharmacy, Kumarapalayam, Tamilnadu, India. Her field of doctoral and research work is primarily on herbal phytoconstituents and medicines for neurodegenerative disorders. She has published 20 research articles in various national and international journals and has received many best poster and oral presentation awards, young scientist award in various national and international conferences.
Abstract:
Biography:
Abstract:
Eman Khalifa
Alexandria University, Egypt
Title: Biodiversity of toxigenic fungi in livestock and poultry feedstuffs in Egypt
Time : 12:20-12:50

Biography:
Abstract:
Pranab Roy
HaldiaInst of Technology, India
Title: Characterization of plant growth promoting bacteria isolated from nodule co-infecting a single leguminous plant

Biography:
Abstract:
Sarita Manandhar
Tribhuvan University, Nepal
Title: Study of antibiotic susceptibility pattern and biofilm formation among staphylococci isolated from tertiary care hospital

Biography:
Abstract:
Sarita Manandhar
Tribhuvan University, Nepal
Title: Study of antibiotic susceptibility pattern and biofilm formation among staphylococci isolated from tertiary care hospital
Time : 13:40-14:10

Biography:
Abstract:
Pranab Roy,
HaldiaInst of Technology, India
Title: Characterization of plant growth promoting bacteria isolated from nodule co-infecting a single leguminous plant
Time : 14:40-15:10

Biography:
Abstract:
Ilknur Tuncer,
Dokuz Eylul University, Turkey
Title: Phylogenetic diversity, antibiotic susceptibility and enzymatic activities of bacterial isolates from sediments of Eastern Mediterranean Sea
Time : 15:20-15:50

Biography:
Abstract:
Gulseren Aktas
Istanbul University, Turkey
Title: Activity of Vancomycin Combined with Linezolid and with Dalbavancin against Clinical VRE Strains
Time : 15:50-16:20

Biography:
Abstract:
Sumon Ghosha
Chittagong Veterinary and Animal Sciences University, Bangladesh
Title: Trends and Clinico -epidemiological features of HUMAN RABIES cases in Bangladesh 2006-2017

Biography:
Communicable Disease Control, Directorate General of Health Services, Ministry of Health and Family Welfare, Bangladesh
Abstract:
Background:
Bangladesh has reduced the death of rabies case-patients by 50% over the last few years. Clinical and epidemiological knowledge of human rabies is crucial to direct further research investigation and to implement disease control measures.
Methods: We used data from the rabies record book of the Infectious Disease Hospital (IDH), Dhaka, Bangladesh from 2006 to 2017. We reviewed the medical records of 422 patients reported from 2011 to 2015 who had a clinical diagnosis of rabies.
Results: We found a strong trend of declining human rabies cases in Bangladesh from 2006 to 2017 (p<0.001 and tau = -0.85). In Sen’s slope test we found, over the 12 year period the slope changes at the rate of 11.45 (95% CI: -14.00 to -8.00) annually (p<0.001). The declining trend of human rabies cases can be explained by the number of dogs being vaccinated against rabies during 2011 to 2017 (adjusted R2=0.55). Dogs with unknown vaccination status, comprised the majority of exposures (n=380, 90%) followed by cat (n=24, 6%), jackal (n=12, 3%) and mongoose (n=6, 1%). The majority (n=327, 78%) of the victims sought treatment from traditional healers and 12% (n=51) received post-exposure prophylaxis (PEP). There was a difference in incubation times between the sites of bites in which the patients were exposed. The shortest, longest and median reported incubation periods were 11, 1080 and 52 days respectively. Cases with a wound on the head and neck appeared to have a shorter incubation time than those with wounds at other sites. Clinical symptoms included hydrophobia (97%), acrophobia (84%), photophobia (10%), and hypersalivation (7%). The case-fatality rate was 100%.
Yeganeh Rezaee
Research Institute of Forests and Rangelands, Iran
Title: Applied and natural ways to controlling MICROBIOLOGY and BACTERIOLOGY growth in Iran

Biography:
I am Iranian scientist. I did my PhD. and Research work on Photochemistry or chemistry (Ag). And up to now my personal life for doing research with our co-worker on cultivation, extraction, formulation and produce natural products out of medicinal and Aromatic plants. So we designed research apparatuses and pilot of essential oil distillation and herbal extraction. Published 6 Book in Persian language and international of two chapters of bio-activity on herbal extracts or essential oil. Finish lots of thesis with different topics on Msc. and PhD. with my students. Publish many papers on this topic in national and international (ISI) journals. Know my position are as scientist in Research Institute Forests and Rangelands (RIFR) -Tehran-IRAN, Chairman of "Union Medicinal plants of Iran" (UMPI) and chair member of "Iranian medicinal plants society" (IMPS). I got three national award abut selected and important medicinal plant as natural product or instead of synthetic medicine and bio-logical uses. And so on…
Abstract:
The increasing demand in finding novel natural compounds or product in the world has diverted scientist’s attention toward of this sources. The very diverse environmental conditions and the rich variety of natural resources made it possible for the Iranians to create a great and flourishing civilization. Iran has hi ranks in medicinal and aromatic plant in natural resource and cultivated manner, traditional knowledge and producing of natural products in the world spatially in Middle East. Iran also is very famous in producing essential oil and water extraction from national plants spatially Rosa damacena, menthe spp and Thym spp. The methods of extraction in Rosa damascena cultivated in extensive zone of Iran are shown different constitution. All Samples were analyzed by GC and GC/MS. The main constituents of oil extracted traditionally, samples were extracted by two hydro distillation method which designed by authors in Research Institute of Forests and Rangelands. Main components of essential oils were citronellol, cis-p-menth -2-en-1-ol and geraniol. These compounds and others are very popular in producing drug anti-cancer, anti-bacterial out of that and we should take care of over doses or toxicology. From my research work, going to presenting the natural constituents and traditionally patients consuming herbal applied and natural ways to controlling different Bacteria growing , as every scientist now reach to the point most of microbes and fungi or…. Become resistant to chemicals uses. So in this way we selected plants and main compounds out of them and got it best results. So it is necessary for a protected herbals and product to be provided with information on its ingredients, indications, dosage, pharmacology, contraindications and possible side effects before a rational decision on its use.
Abdullah Al-Mamari
Edna Adan University, Somaliland
Title: Prevalence of Bacterial Cerebrospinal Meningitis in Infants Attending Federal Medical Center BIRNIN KEBBI And INVITRO ANTIBIOTIC Sensitivity Test

Biography:
Studies of oral fungal have indicated that prevalence of Candida was significantly higher in diabetics both in healthy controls and complete denture wearers compared to non -diabetics. The aims of study was to determine the prevalence oral Candidiasis among both diabetics and non–diabetics patients and evaluate the contribution of risk factors with the prevalence and distribution of Candida species with oral Candidiasis.
Abstract:
The present investigation was carried out in the department of medical microbiology and clinical samples were collected through regular visits to three main hospitals Al-Noor, Al-Amean, Al-Thawra and diagnosis was in the ALFA medical laboratory in Ibb City during period from January into November 2016 .In the current study 70 oral swabs samples were collected from surfaces of the upper of oral cavity and denture of all patients and then placed into a test tube containing 5ml subouraud's dextrose broth. The positive sample was processed for Gram's staining, Germ tube test, Chlamydospores test, Carbohydrate utilization medium examination and Chromagar test was made for differentiation between Candida a species. The results in this study showed that prevalence of Candida oral infection in Ibb City was 36(52%) positive with oral candidiasis out of 70 samples studied while, 34(48%) of patients studied were negative with oral candidiasis. On the other hand, the results also showed the distribution of oral Candida infection among diabetes and non-diabetes patients was 29(41%) and 7(11%) respectively, this results concordant with many previous studies. Candida albicans was the most prevalent types of Candida a species in this study. The results in current investigation also showed a significantly relationship between crews users, diabetes patients, age and oral candidiasis.
Musa Galadima
Kebbi State University of Science and Technology, Nigeria
Title: Prevalence of Bacterial Cerebrospinal Meningitis in Infants Attending Federal Medical Center BIRNIN KEBBI And INVITRO ANTIBIOTIC Sensitivity Test

Biography:
Abstract:
A microbiological study was carried out on infants with suspected cases of cerebrospinal meningitis at Federal Medical Center Birnin Kebbi between February and June, 2017. Sixty six specimens of Cerebrospinal fluid (CSF) were obtained and examined using both microscopic and cultural methods for bacterial pathogens. Results obtained showed that only six (9.09%) of the specimens examined were positive for bacterial pathogens. The bacteria observed were Streptococcus pneumonia, Heamophilus influenza, Neisseria meningitides and Escheria coli. Invitro antibiotic sensitivity test carried on these isolated bacteria indicates that Ceftriaxone, Zinnacef and Gentamycin were active against all the isolates while Ciprofloxacin was active against E. coli. The result showed high prevalence of bacterial meningitis in contrast to its low incidence reported by many workers. There is the need to mount viral screening of CSF in addition to bacterial screening.
Murtala Saadu
Kebbi State University of Science and Technology, Nigeria
Title: synergistic effect of alkaloid and saponin of synergistic effect of ALKALOID and SAPONIN of Vernonia amygdalina on Escherichia coli

Biography:
Abstract:
The study was aimed at investigate quantitatively phytochemical constituents of the leaf extract of Vernonia amygdalina, and determine the Synergistic effect of Alkaloid and Saponin compound of the plant extract against E. coli. The antibacterial activities of the alkaloid, saponin and their synergistic effect were carried out at varying concentration of 50, 25, 12.5 and 6.25mg/ml, using agar well diffusion method. The result of this study indicated the, ethanolic extract shows higher anti-bacterial activity at 100mg/ml concentration (11.29mm) compared to aqueous extract (9.24mm) against the test bacteria. It also showed that the anti-bacterial activity of the alkaloid content of the Vernonia amygdalina at 100mg/ml (11.01mm) was better than that of saponin (9.32mm) while the synergistic effect of alkaloid and saponin at concentration 100mg/ml (15.41mm). Were better than the individual compounds the result indicates the possibility of using the alkaloid and saponin compounds synergistically in the treatment of disease associated with E.coli.