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Day 3 : November 13, 2018

Bacterial Diseases | Bacterial Epidemiology | Bacterial Pathogenesis | Plant Bacteriology | Bacterial Morphology & Metabolism | Bacterial Identification Method

Session Introduction

Eman Khalifa,

Alexandria University, Egypt

Title: Biodiversity of toxigenic fungi in livestock and poultry feedstuffs in Egypt

Biography:

Eman Khalifa is a Faculty of Veterinary Medicine, Alexandria University, Matrouh Branch and Acting Dean of Faculty of Veterinary Medicine, Alexandria University, Matrouh Branch and Assistant Professor of Microbiology and Immunology and Manager of Information Technology (IT Unit) Expert in Biotechnology, Food Microbiology, animal Microbiology, Molecular diagnostic Microbiology and Had the first record in Egypt and worldwide for isolation and identification of Trichoderma pseudokoningii Rifai from Egyptian immunocompromised cattle with Mycobacterium bovis infection with international record from Mycological Center, Assiut

University, and published in Nature Precedings: hdl:10101/npre.2012.6928.1 : Posted 23 Feb 2012.

Abstract:

This study was aimed to investigate the prevalence toxigenic fungi and mycotoxins, a total no. of 90 samples representing various types of animal and poultry feedstuffs were collected from different localities in Egypt: Alexandria, Beheira, New Valley, Assiut and Cairo, The results showed that 78 (86.7%) of samples were contaminated with different fungal species, the fungal load was ranged from 0.4 to 12×10³ cfu/gram among different samples, Aspergillus was the most common genus being isolated from all mycological positive samples with total count of 101,900 colonies per gram matching 83.84% of total fungal

population. 16 species of Aspergillus which are A. flavus and A. niger were the most common colonizing 72.2% and 56.6% of samples matching 15.8% and 41.7% of total fungal count. Penicillium appeared in 50% of samples accounting for 13.1% of total fungal population. Also in the present study 13 feedstuff samples were analyzed for mycotoxin contamination. These samples

were mycological positive yielding low (40-600 cfu/g) or high number of fungal colonies (1280-4920 cfu/g). A. flavus and A. niger were the dominant species in these samples, aflatoxin B1 at level of 0.851 and 1.363 μg/kg were detected in 2 samples, respectively. Aflatoxin B2 at a level of 0.479 μg/kg was also detected in 1 sample. Levels of Aflatoxins in the positive samples

(AFB1 and AFB2) are below the recommended limit for animal feedstuff ingredients for beef cattle (20 μg/kg). From previously mentioned data, it was clear that feedstuffs of animal and poultry in Egypt represented hazardous source of toxigenic fungi which harm animal and poultry health, decrease their production, and could be serious sources of human illness, so regular

screening in animal and poultry feedstuffs in Egypt is a good practice in order to control and overcome their presence and

dangers.

Fatoba Johnson Sunday

University of Benin, Nigeria

Title: Enterococcus Faecalis As Nosocomial Pathogen

Biography:

Abstract:

Shivatra Talchai,

Medical Innovative Technology, Singapore

Title: Expansion of High Quality Mesenchymal Stem Cells On Xeno Free Cell Revtm Msc Exhq For Therapeutic Applications

Biography:

Zher Min Tan BS, Priyatharshini Murugan MS, Shivatra Talchai PhD, Veerapol Khemarangsan MD, SMIT Stem Cell – Singapore Medical Innovative Technology Pte Ltd, 50 Science Park Road, #04-03, The Kendall, Singapore 117406

Abstract:

Multipotent mesenchymal stem cells (MSCs) that have self-renewal properties and can differentiate into adipocytes, osteoblasts, chondrocytes and others. MSCs have been found to have immunomodulatory effects. Thus, MSCs represent an ideal source of stem cells for tissue engineering applications.

To ensure their quality, biocompatibility and consistency for clinical applications, it is essential for MSCs cultured ex vivo to retain of their self-renewal and differentiation capacities. Traditionally, fetal bovine serum (FBS), dubbed standard media (STD), is used as a supplement to support cells in in vitro culture. However, this is disadvantageous as FBS is posing clinical risks such as viral or prion contamination and immune rejection, as well as affecting the differentiation potential of MSCs. To produce high quality MSCs for clinical use, sera- and animal-free media are being developed. We therefore formulated a human platelet lysate-based, serum-free media, MSC ExHQ Cell Rev^TM media that can be used to expand MSCs while retaining their multipotent differentiation potentials. Here, we compare the properties of MSCs cultured in standard media (STD) versus in MSC ExHQ Cell Rev^TM media. We found that standard media leads to overexpression of MSC negative marker, CD19 while ExHQ media does not. Therefore, we suggest that MSC ExHQ Cell Rev^TM media is more appropriate media for high quality MSCs as well as for later clinical applications.

Christoper John N Tibayan

Ospital Ng Makati, Philippines

Title: Second Day Sequential Organ Failure Assessment (SOFA) Score as Predictor in 30â€“Day Hospital Mortality among Filipino Adult Patients who presented with Sepsis at the Emergency Department

Biography:

Sepsis-3 Guidelines promoted the new definition and prognostication scheme among patients with sepsis using the Sequential Organ Failure Assessment (SOFA) Scoring System. The study determined the discriminative power of the 2nd day SOFA scoring system in predicting 30-day hospital mortality among adult Filipino patients who presented with sepsis and suggested adjustment on the applicability of SOFA scoring system in a resource limited government hospital in the Philippines. We evaluated 107 adults with sepsis presenting at the emergency department from June to August 2017 at Ospital Ng

Makati, Philippines. Receiving operating characteristic curves were generated to determine optimal cut off scores of SOFA in

predicting 30-day mortality. Binary logistic regression was performed to determine the association of the SOFA derivatives with

hospital mortality.

Abstract:

Gomathi Swaminathan

JKKN College of Pharmacy, India

Title: Phytochemical Analysis and neuroprotective activity of Pedalium murex linn Leaves against bacterial cell wall Content lipopolysacchride-induced endotoxemia

Biography:

Mrs. Gomathi Swaminathan, M.Pharm., is perusing part-time Ph.D in The Tamil Nadu Dr. M.G.R Medical University, Chennai, Tamilnadu, India and is a full-time faculty of J. K. K. Nattraja College of Pharmacy, Kumarapalayam, Tamilnadu, India. Her field of doctoral and research work is primarily on herbal phytoconstituents and medicines for neurodegenerative disorders. She has published 20 research articles in various national and international journals and has received many best poster and oral presentation awards, young scientist award in various national and international conferences.

Abstract:

Neurodegenerative diseases (ND) are threatening worldwide. Huge number of behavioural changes are associated with ND such as depression, anxiety, memory loss, stress and so on. Effective drugs need to cure the ND from nature. In this present study, we had evaluated the phytochemical analysis and neuroprotective activity of Pedalium murex Linn (PM) leaves against bacterial cell wall content Lipopolysaccharide (LPS)-induced endotoxemia in rats. Two compounds were isolated from 90% v/v of ethanol extract of Pedalium murex Linn (EEPM) named as PM I, PM II. Spectral data of isolated compounds strongly suggested that PM I showed the structural similarities with 4´, 5, 7- trihydroxy flavone may be Apigenin and PM II showed the structural similarities with 3,5,7-Trihydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one which may have the presence of kaempferol. The data of our present study revealed that LPS significantly decreases the food, water intake and body mass which was recovered EEPM treated rats. EEPM attenuated or completely abolished the symptoms of depression and anxiety. Antioxidant enzymes includes SOD, CAT, GR, GPX levels were decreased in LPS-treated rats, which was recovered by EEPM treated rats. Histopathological results also support the neuroprotective effect of EEPM against LPS-induced neurodegeneration. We may conclude that EEPM may be a potential candidate for LPS-induced brain damage which may be attributed to the presence of potent antioxidants in EEPM

Donald Kuah

Sydney Sportsmed Specialists, Australia

Title: Second Day Sequential Organ Failure Assessment (SOFA) Score as Predictor in 30â€“Day Hospital Mortality among Filipino Adult Patients who presented with Sepsis at the Emergency Department

Biography:

Donald Kuah is an experienced Sports and Exercise Physician specialist and one of the founding practitioners at Sydney Sports Medicine Centre in Olympic Park. He has been the Chief Medical Officer to the Wests Tigers NRL team (2003-2013), is the current Chairman of the NRL Medical Advisory Panel and is on the Olympic Winter Institute of Australia medical panel. He was the NSW Institute of Sport Medical Director from 2000-2012, being responsible for the state’s top 700 elite athletes and remains involved with treating NSWIS athletes. Dr Kuah is involved in teaching at many levels and also heavily involved in clinical research. He

has previously been the State Training Coordinator for the Australasian College of Sport and Exercise Medicine and continues to be a Clinical Training Supervisorand an Examiner for the College. His interest areas lie in treatment of tendon pathologies, osteoarthritis, hamstring origin pathologies and also in the area of

regenerative medicine.

Abstract:

Osteoarthritis (OA) is a debilitating condition with no known cure or disease-modifying treatments available. Stem cell therapies may have the potential to halt OA disease progression and repair and regenerate affected joints. Bone Marrow (BM) and adipose-derived (AD) MSCs are being trialed for multiple diseases including OA but the majority of research has involved use of autologous cells (rather than allogeneic). A brief review of the literature will be presented. Two studies of knee OA patients receiving allogeneic BM-MSCs concluded MSCs were safe but varying efficacy results were reported. One study reported significant decreases in pain and encouraging MRI results in the MSC treated group. The second study observed no significant changes in objective and subjective pain measurements and no cartilage improvements by MRI. Allogeneic MSC safety has also been demonstrated in a cartilage defect study and in a study delivering the MSCs post partial meniscectomy. These studies reported improvements in pain and MRI measurements in patients that received MSCs. The STEP (Safety, tolerability and Efficacy of Progenza) Trial was a first-in-human, Phase I, randomised, double-blind, placebo-controlled single

ascending dose study of Progenza (PRG) for OA. PRG is composed of in vitro expanded AD-MSCs derived from human donor

adipose tissue combined with cell culture supernatant, frozen in ready to inject vials. The primary objective was to evaluate the

safety and tolerability of PRG administered via a single intra-articular injection. Secondary objectives included investigating the

effect of PRG on pain, function and joint structures in the study knee, quality of life and relevant serum and urine biomarkers.

Twenty patients (40-65 years; Kellgren-Lawrence grade 1-3 knee OA and 35-90mm visual analogue scale pain score) were

treated in two cohorts of 10 patients with a 12 month follow-up. Cohort 1: 3.9 million cells (n=8) or placebo (n=2) and cohort

2: 6.7 million cells (n=8) or placebo (n=2). All patients experienced at least 1 treatment emergent adverse event. Most were

mild and unrelated to the Investigational Product (IP). No serious adverse events were reported, and no patients withdrew

from the study due to an AE. VAS pain scores (Fig. 1) and WOMAC pain subscale scores significantly improved from baseline

at all timepoints in the PRG groups (p < 0.05) whilst only a marginal non-statistically significant improvement was observed

in the placebo group. Lateral tibial cartilage volume was preserved in the PRG 3.9M group whilst the placebo group showed a

statistically significant cartilage loss. PRG was safe and well tolerated when administered as a single intra-articular injection to

patients with symptomatic knee OA. Improvements in knee structure outcomes demonstrates the potential for PRG to have a

disease modifying effect in OA joints which warrants further investigation.

Eman Khalifa

Alexandria University, Egypt

Title: Biodiversity of toxigenic fungi in livestock and poultry feedstuffs in Egypt

Time : 12:20-12:50

Biography:

Eman Khalifa is a Faculty of Veterinary Medicine, Alexandria University, Matrouh Branch and Acting Dean of Faculty of Veterinary Medicine, Alexandria University,Matrouh Branch and Assistant Professor of Microbiology and Immunology and Manager of Information Technology (IT Unit) Expert in Biotechnology, Food Microbiology, animal Microbiology, Molecular diagnostic Microbiology and Had the first record in Egypt and worldwide for isolation and identification of Trichoderma pseudokoningii Rifai from Egyptian immunocompromised cattle with Mycobacterium bovis infection with international record from Mycological Center, Assiut University, and published in Nature Precedings: hdl:10101/npre.2012.6928.1 : Posted 23 Feb 2012.

Abstract:

screening in animal and poultry feedstuffs in Egypt is a good practice in order to control and overcome their presence and

dangers.

Pranab Roy

HaldiaInst of Technology, India

Title: Characterization of plant growth promoting bacteria isolated from nodule co-infecting a single leguminous plant

Biography:

Pranab Roy has completed his Post-Doctoral Research from Yale University School of Medicine and University of Connecticut Health Centre, USA working onadvanced Molecular Biology. He has joined as Lecturer in Bose Institute where he characterized a novel repetitive DNA sequence by molecular cloning and

sequencing when these techniques were just being introduced in India in mid-80’s. He also joined industrial R&D to set up the Genetic Engineering Unit at

Hindustan Lever Research Centre, Mumbai. He is currently working at Haldia Institute of Technology, where his research interest was with plant growth promotingrhizobacteria from root nodules from Fenugreek (Methi), a leguminous plant

Abstract:

The green revolution ushered in a growth in the production of food crops in India. From a net importer of food grains, India became self-sufficient increasing the total production from 50 million metric tons in mid-sixties to 200 million metric tons in mid-eighties. This was possible due to higher yielding and hybrid varieties of seeds, increased input of chemical

fertilizers and insecticides or pesticides. However, the pitfalls of high usage of chemical fertilizers were deterioration in the quality of agricultural soil. The natural microbes present in the rhizospheric soil died due to excess chemicals and the depletion of organic carbon content. The water retention capacity was also adversely affected due to lower porosity of the soil, sometimes

the soil became acidic causing lower productivity of plants. To overcome all these problems, the use of organic manure and bio-fertilizers were introduced. These are beneficial to the crop plants due to the following properties like nitrogen fixation, inorganic phosphate solubilization, production of siderophore and production of ACC deaminase. Nitrogen fixation by symbiotic microorganisms like Rhizobium, occurring in the root nodules of leguminous plants have been reported long back. In our studies, aseptic root nodules of Fenugreek (Methi) were crushed to isolate a variety of nitrogen fixing microbes. Though microbiological and biochemical studies indicated these mucoid colonies all Gram negative with different morphologies to the

Rhizobium species, sequencing of 16s rRNA genes and molecular phylogeny showed three of the isolates (1) R1 Enterobactercloacae (KX687556) (2) R10 Pantoea dispersa (KX687557) and (3) R12 Enterobacter ludwigii (KX687554). The temperatureand salinity tolerance of these cultures were studied to increase these properties of abiotic stress tolerance. UV mutagenesiswas tried, varying the time of exposure of the plated cultures to UV radiation, the survivors (>1% of initial population) werescreened for growth at 0.5 M NaCl and at 44 °C. The selected mutants of R1, R10 and R12 were studied for the plant growthpromoting activities like IAA (growth hormone) production, siderophore production and phosphate solubilization. Finally,the effects of the isolated bacteria and mutants of each and a consortium of all three were pre-treated with methi seeds andgerminated in pots to find out their effects on the growth of the plants. These are being analyzed after 40 days of growth in the

soil.

Sarita Manandhar

Tribhuvan University, Nepal

Title: Study of antibiotic susceptibility pattern and biofilm formation among staphylococci isolated from tertiary care hospital

Biography:

Staphylococci are notorious human pathogens that cause variety of diseases ranging from minor skin infections to chronic systemic infections such as osteomyelitis, endocarditis and biofilm associated infections of in dwelling devices. These infections have also the potential to spread both in health care facilities and communities eventually causing minor outbreaks. A multitude of factors including but not limited to antibiotic resistance, biofilm production and formation of antibiotic tolerant persister cells contribute to treatment failures in such staphylococcal infections.

Abstract:

A total of 375 Staphylococci were isolated from clinical samples received in a tertiary care hospital of Nepal during 2015-2017 were included to study antibiotic susceptibility pattern, biofilm formation and persister cells development. Standard microbiological procedure was used to identify staphylococcal species and CLSI guideline was followed to determine antibiotic susceptibility pattern. Biofilm production was detected using both phenotypic and genotypic methods. Among 375 Staphylococcal isolates, 161 isolates were identified as S. aureus comprising 81% methicillin resistant

and 19% methicillin sensitive S. aureus and remaining coagulase negative Staphylococcus spp. While most of the isolates were resistant to Penicillin, these were sensitive towards Chloramphenicol, Tetracycline and Clindamycin. Among different phenotypic assays used for detection of biofilm formation, tissue culture method detected biofilm production in 22.1% isolates followed by tube method and Congo red agar method which detected only 16.8% and 5.3%, respectively. On further verification, PCR detected ica genes in 23% of staphylococcal isolates thus revealing the biofilm formation in these samples.

In-vitro persister assay is undergoing to study the causal link between persister cells and treatment failures in these isolates

Sarita Manandhar

Tribhuvan University, Nepal

Title: Study of antibiotic susceptibility pattern and biofilm formation among staphylococci isolated from tertiary care hospital

Time : 13:40-14:10

Biography:

Abstract:

A total of 375 Staphylococci were isolated from clinical samples received in a tertiary care hospital of Nepal during 2015-2017 were included to study antibiotic susceptibility pattern, biofilm formation and persister cells development. Standard microbiological procedure was used to identify staphylococcal species and CLSI guideline was followed to determine antibiotic susceptibility pattern. Biofilm production was detected using both phenotypic and genotypic methods.Among 375 Staphylococcal isolates, 161 isolates were identified as S. aureus comprising 81% methicillin resistant and 19% methicillin sensitive S. aureus and remaining coagulase negative Staphylococcus spp. While most of the isolates were resistant to Penicillin, these were sensitive towards Chloramphenicol, Tetracycline and Clindamycin. Among different

phenotypic assays used for detection of biofilm formation, tissue culture method detected biofilm production in 22.1% isolates followed by tube method and Congo red agar method which detected only 16.8% and 5.3%, respectively. On further verification, PCR detected ica genes in 23% of staphylococcal isolates thus revealing the biofilm formation in these samples. In-vitro persister assay is undergoing to study the causal link between persister cells and treatment failures in these isolates.

Pranab Roy,

HaldiaInst of Technology, India

Title: Characterization of plant growth promoting bacteria isolated from nodule co-infecting a single leguminous plant

Time : 14:40-15:10

Biography:

sequencing when these techniques were just being introduced in India in mid-80’s. He also joined industrial R&D to set up the Genetic Engineering Unit at

Abstract:

The green revolution ushered in a growth in the production of food crops in India. From a net importer of food grains,India became self-sufficient increasing the total production from 50 million metric tons in mid-sixties to 200 million metric tons in mid-eighties. This was possible due to higher yielding and hybrid varieties of seeds, increased input of chemical

was tried, varying the time of exposure of the plated cultures to UV radiation, the survivors (>1% of initial population) were screened for growth at 0.5 M NaCl and at 44 °C. The selected mutants of R1, R10 and R12 were studied for the plant growth promoting activities like IAA (growth hormone) production, siderophore production and phosphate solubilization. Finally, the effects of the isolated bacteria and mutants of each and a consortium of all three were pre-treated with methi seeds and germinated in pots to find out their effects on the growth of the plants. These are being analyzed after 40 days of growth in the

soil.

Ilknur Tuncer,

Dokuz Eylul University, Turkey

Title: Phylogenetic diversity, antibiotic susceptibility and enzymatic activities of bacterial isolates from sediments of Eastern Mediterranean Sea

Time : 15:20-15:50

Biography:

Ilknur Tuncer has received two BSc degrees in Chemistry and Genetics from Bogazici University, Turkey, MSc degree in Marine Sciences from Bangor University, UK and PhD degree in Marine Sciences from Dokuz Eylul University, Turkey. She has several books and publications. She is an Editorial Member for several scientific journals. Her current research interests are marine environment, microbiology, phylogeny, species-area relations, human health and medicine.

Abstract:

Phylogenetic diversity of isolated bacteria and biogeochemical variability of sediments in shallow and deep basins of Eastern Mediterranean Sea were studied. According to 16S rRNA gene sequence analysis, the strains isolated from the study area were belonged to the Firmicutes, Gammaproteobacteria and Actinobacteria. The present study provided highly diverse bacterial strains of 14 different families especially with probability of representing new taxa in addition to the dominant genus bacillus in each sediment samples. The actinomycetes were tested physiologically using commercial kits and found

that they utilized proteins rather than carbohydrates. Geochemical parameters of the sediments together with enzymatic activity results of the strains underlined the nitrogen limitation in the area. The highest antibiotic resistance for the Firmicutes and Gammaproteobacteria strains was obtained from coastal sediments compared to deep basins supported by a positive correlation between sediment size and antibiotic resistance. The influence of environmental parameters was observed as the variability of bacterial community composition such that phylogenetic diversity was much higher in higher taxa for shallower

regions, especially in North Aegean Sea as compared to deep basins of Eastern Mediterranean Sea as supported in hierarchical cluster analysis of the isolates and statistical analysis including principle component analysis.

Gulseren Aktas

Istanbul University, Turkey

Title: Activity of Vancomycin Combined with Linezolid and with Dalbavancin against Clinical VRE Strains

Time : 15:50-16:20

Biography:

Abstract:

A total of 30 randomly-selected clinical VRE strains were included in the study. Each strain was isolated from different patients who were admitted to the different clinics of Istanbul University, Istanbul Faculty of Medicine’s Hospital. Bacterial identifications of the strains were undertaken using conventional routine methods. The antibiotics used in the study were

vancomycin, linezolid and dalbavancin. Resistance to agents tested was investigated by micro-broth dilution assay as described by the Clinical and Laboratory Standards Institute (CLSI). They were prepared in accordance with CLSI proposals. Cation Adjusted Mueller-Hinton Broth (CAMHB) was used for all experiments. Additionally, in the tests for dalbavancin, CAMHB

with 0.002% final concentration of polysorbate 80 was used. The inoculum of strain was adjusted to approximately 5×105 cfu/mL in the wells. The Minimum Ä°nhibitory Concentration (MIC) was defined as the lowest concentration of antibiotic giving complete inhibition of visible growth and interpreted in accordance with CLSI guidelines. Quality control of the test procedures was monitored via testing of the reference strains S. aureus ATCC 29213 and E. faecalis ATCC 29212. In vitro activities of antibiotics in combination were assessed using the micro-broth checker board technique. The Fractional Ä°nhibitory Concentration (FIC) indexes were calculated using the following formula: FICI=FICA+FICB. The FICI was interpreted as synergism, FICI≤0.5; additive/indifference, FICI δ0.5-≤4; antagonism, FICIδ4.

Sumon Ghosha

Chittagong Veterinary and Animal Sciences University, Bangladesh

Title: Trends and Clinico -epidemiological features of HUMAN RABIES cases in Bangladesh 2006-2017

Biography:

Communicable Disease Control, Directorate General of Health Services, Ministry of Health and Family Welfare, Bangladesh

Abstract:

Background:

Bangladesh has reduced the death of rabies case-patients by 50% over the last few years. Clinical and epidemiological knowledge of human rabies is crucial to direct further research investigation and to implement disease control measures.

Methods: We used data from the rabies record book of the Infectious Disease Hospital (IDH), Dhaka, Bangladesh from 2006 to 2017. We reviewed the medical records of 422 patients reported from 2011 to 2015 who had a clinical diagnosis of rabies.

Results: We found a strong trend of declining human rabies cases in Bangladesh from 2006 to 2017 (p<0.001 and tau = -0.85). In Sen’s slope test we found, over the 12 year period the slope changes at the rate of 11.45 (95% CI: -14.00 to -8.00) annually (p<0.001). The declining trend of human rabies cases can be explained by the number of dogs being vaccinated against rabies during 2011 to 2017 (adjusted R²=0.55). Dogs with unknown vaccination status, comprised the majority of exposures (n=380, 90%) followed by cat (n=24, 6%), jackal (n=12, 3%) and mongoose (n=6, 1%). The majority (n=327, 78%) of the victims sought treatment from traditional healers and 12% (n=51) received post-exposure prophylaxis (PEP). There was a difference in incubation times between the sites of bites in which the patients were exposed. The shortest, longest and median reported incubation periods were 11, 1080 and 52 days respectively. Cases with a wound on the head and neck appeared to have a shorter incubation time than those with wounds at other sites. Clinical symptoms included hydrophobia (97%), acrophobia (84%), photophobia (10%), and hypersalivation (7%). The case-fatality rate was 100%.

Yeganeh Rezaee

Research Institute of Forests and Rangelands, Iran

Title: Applied and natural ways to controlling MICROBIOLOGY and BACTERIOLOGY growth in Iran

Biography:

I am Iranian scientist. I did my PhD. and Research work on Photochemistry or chemistry (Ag). And up to now my personal life for doing research with our co-worker on cultivation, extraction, formulation and produce natural products out of medicinal and Aromatic plants. So we designed research apparatuses and pilot of essential oil distillation and herbal extraction. Published 6 Book in Persian language and international of two chapters of bio-activity on herbal extracts or essential oil. Finish lots of thesis with different topics on Msc. and PhD. with my students. Publish many papers on this topic in national and international (ISI) journals. Know my position are as scientist in Research Institute Forests and Rangelands (RIFR) -Tehran-IRAN, Chairman of "Union Medicinal plants of Iran" (UMPI) and chair member of "Iranian medicinal plants society" (IMPS). I got three national award abut selected and important medicinal plant as natural product or instead of synthetic medicine and bio-logical uses. And so on…

Abstract:

The increasing demand in finding novel natural compounds or product in the world has diverted scientist’s attention toward of this sources. The very diverse environmental conditions and the rich variety of natural resources made it possible for the Iranians to create a great and flourishing civilization. Iran has hi ranks in medicinal and aromatic plant in natural resource and cultivated manner, traditional knowledge and producing of natural products in the world spatially in Middle East. Iran also is very famous in producing essential oil and water extraction from national plants spatially Rosa damacena, menthe spp and Thym spp. The methods of extraction in Rosa damascena cultivated in extensive zone of Iran are shown different constitution. All Samples were analyzed by GC and GC/MS. The main constituents of oil extracted traditionally, samples were extracted by two hydro distillation method which designed by authors in Research Institute of Forests and Rangelands. Main components of essential oils were citronellol, cis-p-menth -2-en-1-ol and geraniol. These compounds and others are very popular in producing drug anti-cancer, anti-bacterial out of that and we should take care of over doses or toxicology. From my research work, going to presenting the natural constituents and traditionally patients consuming herbal applied and natural ways to controlling different Bacteria growing , as every scientist now reach to the point most of microbes and fungi or…. Become resistant to chemicals uses. So in this way we selected plants and main compounds out of them and got it best results. So it is necessary for a protected herbals and product to be provided with information on its ingredients, indications, dosage, pharmacology, contraindications and possible side effects before a rational decision on its use.

Abdullah Al-Mamari

Edna Adan University, Somaliland

Title: Prevalence of Bacterial Cerebrospinal Meningitis in Infants Attending Federal Medical Center BIRNIN KEBBI And INVITRO ANTIBIOTIC Sensitivity Test

Biography:

Studies of oral fungal have indicated that prevalence of Candida was significantly higher in diabetics both in healthy controls and complete denture wearers compared to non -diabetics. The aims of study was to determine the prevalence oral Candidiasis among both diabetics and non–diabetics patients and evaluate the contribution of risk factors with the prevalence and distribution of Candida species with oral Candidiasis.

Abstract:

The present investigation was carried out in the department of medical microbiology and clinical samples were collected through regular visits to three main hospitals Al-Noor, Al-Amean, Al-Thawra and diagnosis was in the ALFA medical laboratory in Ibb City during period from January into November 2016 .In the current study 70 oral swabs samples were collected from surfaces of the upper of oral cavity and denture of all patients and then placed into a test tube containing 5ml subouraud's dextrose broth. The positive sample was processed for Gram's staining, Germ tube test, Chlamydospores test, Carbohydrate utilization medium examination and Chromagar test was made for differentiation between Candida a species. The results in this study showed that prevalence of Candida oral infection in Ibb City was 36(52%) positive with oral candidiasis out of 70 samples studied while, 34(48%) of patients studied were negative with oral candidiasis. On the other hand, the results also showed the distribution of oral Candida infection among diabetes and non-diabetes patients was 29(41%) and 7(11%) respectively, this results concordant with many previous studies. Candida albicans was the most prevalent types of Candida a species in this study. The results in current investigation also showed a significantly relationship between crews users, diabetes patients, age and oral candidiasis.

Musa Galadima

Kebbi State University of Science and Technology, Nigeria

Title: Prevalence of Bacterial Cerebrospinal Meningitis in Infants Attending Federal Medical Center BIRNIN KEBBI And INVITRO ANTIBIOTIC Sensitivity Test

Biography:

Abstract:

A microbiological study was carried out on infants with suspected cases of cerebrospinal meningitis at Federal Medical Center Birnin Kebbi between February and June, 2017. Sixty six specimens of Cerebrospinal fluid (CSF) were obtained and examined using both microscopic and cultural methods for bacterial pathogens. Results obtained showed that only six (9.09%) of the specimens examined were positive for bacterial pathogens. The bacteria observed were Streptococcus pneumonia, Heamophilus influenza, Neisseria meningitides and Escheria coli. Invitro antibiotic sensitivity test carried on these isolated bacteria indicates that Ceftriaxone, Zinnacef and Gentamycin were active against all the isolates while Ciprofloxacin was active against E. coli. The result showed high prevalence of bacterial meningitis in contrast to its low incidence reported by many workers. There is the need to mount viral screening of CSF in addition to bacterial screening.

Murtala Saadu

Kebbi State University of Science and Technology, Nigeria

Title: synergistic effect of alkaloid and saponin of synergistic effect of ALKALOID and SAPONIN of Vernonia amygdalina on Escherichia coli

Biography:

Abstract:

The study was aimed at investigate quantitatively phytochemical constituents of the leaf extract of Vernonia amygdalina, and determine the Synergistic effect of Alkaloid and Saponin compound of the plant extract against E. coli. The antibacterial activities of the alkaloid, saponin and their synergistic effect were carried out at varying concentration of 50, 25, 12.5 and 6.25mg/ml, using agar well diffusion method. The result of this study indicated the, ethanolic extract shows higher anti-bacterial activity at 100mg/ml concentration (11.29mm) compared to aqueous extract (9.24mm) against the test bacteria. It also showed that the anti-bacterial activity of the alkaloid content of the Vernonia amygdalina at 100mg/ml (11.01mm) was better than that of saponin (9.32mm) while the synergistic effect of alkaloid and saponin at concentration 100mg/ml (15.41mm). Were better than the individual compounds the result indicates the possibility of using the alkaloid and saponin compounds synergistically in the treatment of disease associated with E.coli.

Clinical Diagnostic Bacteriology | Microbial Genomics | Applied Microbiology | Medical Microbiology | Dairy Microbiology | Aquatic Microbiology

Session Introduction

El Hadji Seydou Mbaye

Aristide Le Dantec Hospital, West Africa

Title: Human Papillomavirus Infection in genital Women in four regions

Biography:

Dr. El Hadji Seydou Mbaye was born in 1978 in Kaolack a region of Senegal. During 2008-2013, he persued his PhD in Biology and Human Pathologies with the collaboration of the International Agency for Research on Cancer (IARC) /WHO, Lyon (France); 2006-2007 : Master of Life and Health, Specialty Biology of microorganisms, Virology in Louis Pasteur University of Strasbourg (France); 2005-2006 : Master of Life and Health, option of Immunophysiopathology in Louis Pasteur University of Strasbourg (France); 2004-2005 : License of Biochemistry in Louis Pasteur University of Strasbourg (France); 2002-2004 : General Degree in Sciences and Technologies in University of METZ (France). He was certified by the Federation International of Gynecology Obstetrics (FIGO), the Accreditation Council of Oncology in Europe (ACOE, www.acoe.be), the Institute Catalan of Oncology (ICO) for cervical cancer prevention (Grade 10/10) in support of Continuing Medical Education for physicians. These credits are also recognised as Physician’s Recognition Award (AMA PRA Category 1 credits) by the American Medical Association. He was certified, by the United Nations for Basic Notion of Security on the Ground-Protection, Health and behavior, by the International Agency for Research on Cancer (IARC)/World Health Organization, Lyon (France) for Safety Certificate. He has published 1 Book with a style of philosophical story. Author of the world program against cancer in low and middle incomes countries, he is lead author (first listed) of more than 90 peer-reviewed articles published in reputed journals. He is Editorial Board Member of the International Journal of Immunology, associate membership of the World Society for Virology and also, member of BCNet International Working Group, International Agency for Research on Cancer (IARC)/World Health Organization (WHO).

Abstract:

Cervical cancer is the most frequent cancer among women in Senegal. However, there are few data concerning the HPV types inducing neoplasia and cervical cancers and their prevalence, in the general population of Senegal The aim of this study is to determine the prevalence of HPV infection in Senegalese women aged from 18 years and olderA study was performed on 498 cervix samples collected from healthy women aged 18 and older in Dakar. 438 other samples were collected from three other regions, Thiès, Saint Louis and Louga. The samples were screened for 21 HPV genotypes using an HPV type-specific E7 PCR bead-based multiplex genotyping assay (TS-MPG) which is a laboratory-developed method for the detection of HPVThe prevalence for pHR/HR-HPV in the region of Dakar was 20.68%. HPV 52 (3.21%) was the most prevalent HPV type, followed by HPV 16 (3.01%) and HPV 31 (3.01%). In the regions of Thiès, Louga and Saint Louis, the prevalence for pHR/HR-HPV was 29.19%, 23.15% and 20%, respectively

Virendra Kumar

National JALMA Institute for Leprosy, India

Title: identification of genes involved in biofilm formation in M. AVIUM and M. TUBERCULOSIS MYCOBACTERIA using molecular and electron microscopic tools

Biography:

Research areas mainly infectious diseases like Leprosy, and Tuberculosis I am working in this Institute since last more than 30 years I have done MSc., M.Phil & Ph.D from Agra College, throughout Ist class I am guiding Ph.D,students Project and Training students who wish to do research work in biomedical fields.I got an opportunity to take my basic training by eminent electron microscopistProf. M. Nishiura, from Kyoto University, Japan for more than 2 years. I also privileged to take the training on “Cell Ultra structural and Electron Microscopy” under Prof. B.K.Ghosh, Ruttgers University, New Jersey, U.S.A. in WHO workshop.

Abstract:

Electron microscopy used to investigate the detailed structure and configuration of the mycobacteria which may lead to an understanding the role of infections and in transmitting the diseases. In the natural world more than 99% of bacteria exist as biofilms and according to NIH report more then 68% of all human infections are associated with biofilms formation. Biofilms are slimy, glue-like substance that excreted by bacteria and aggregate on living surface. Biofilms are formed to protect the bacteria from host defences, antibiotics and from harsh environmental conditions. Biofilms are found almost everywhere in nature, including soil, water pipes, and even inside the human body. Many Mycobacteria can develop biofilm, a multicellular structure largely combining bacteria and their extracellular polymeric substances (EPS). The formation of biofilm results in an alternative existence in which microbes ensure their survival in adverse environments. Bacterial biofilms are often associated with infections specially with medical implants such as catheters and other medical devices. Attachment of mycobacteria involved in biofilm formation in the liquid air interface is a complex process, with many variables such as pH, nutrient levels, iron, oxygen, ionic strength and temperature, affecting the outcome.

We had taken two mycobacterial species for study of Mycobacterial biofilm. The isolates were subcultured and characterized biochemically and molecularly. M.avium developed strong amount of biofilm at 30^oC and 42^oC as compared to 37⁰C. M tuberculosis (H37Rv) developed strong biofilm at 37⁰C and no biofilm at 30⁰C and 42⁰C in MB 7H9 media and Sauton’s media. The selected non tuberculosis mycobacteria and H37Rv developed strong biofilm in the presence of OADC enrichment in MB7H9 as well as Sauton’s medium. Isoniazid showed strong inhibited biofilm in fast grower and sensitive isolates. However, Pyrazinamide and Isoniazid inhibited biofilm of M.tuberculosis (H37Rv) and in MDR isolates Ethionamide and Moxifloxacin inhibited biofilm in slow grower and fast grower Mycobacteria. However, many mycobacterial species are known to form biofilms, little is known about either the genetic requirements, patterns of gene expression. In micro array hybridisation we have found that six genes were expressed in M.avium. In M. tuberculosis MDR isolates seven genes were expressed and two genes Rv0359 and Rv3526 were homologus as earlier reported in P. areuginosa and M. avium which might be responsible for biofilm formation.

Fatoba Johnson Sunday

University of Benin, Nigeria

Title: Enterococcus Faecalis As NOSOCOMIAL PATHOGEN

Biography:

I Fatoba Johnson Sunday was born on the 19th of May, 1991. I hail from Aisegba Ekiti Ekiti State Nigeria where I was also born and bred. Being the third of a family of five, it was a privilege that refined my character in teaching me humility and responsibility, and also developing my leadership acumen as I saw my younger ones grow up. I hold a bachelor's degree in Microbiology from Ekiti State university and also Master's degree program in Medical Microbiology (Bacteriology) at the University of Benin, Edo State. Sequel to my passion for academics, I have my passion geared towards reading journals, textbooks, research, periodicals. And this has widened my knowledge in the academic field and has also narrowed my focus in medical microbiology. Towards career development, I currently lecture Communicable disease and immunology at the Grand Royal College of Health Technology, Agbado Ekiti. And my wealth of experience and exposure enables me to present education in its simplest form as I impart knowledge and character to the pupils. Owing to these career trend. I intend to vehemently pursue my academic goals with all diligence as I make contribution to knowledge in my country Nigeria and beyond.

Abstract:

Enterococcus faecalis is Gram-positive, catalase-negative, non-spore-forming, facultative anaerobic bacteria, which usually inhabit the alimentary tract of humans in addition to being isolated from environmental and animal sources. They are able to survive hostile environments, such as in extreme temperature (5-65⁰C), pH (4.5-10.0) and high NaCl concentration, enabling them to colonize a wide range of niches. Depending on the site of infection this organism colonizes, invades the host tissue using their surface adhensin causing pathological changes in the host. Enterococcus faecalis is the leading causative agent of serious nosocomial infections such as urinary tract infection (UTIs), endocarditis, bacteremia, intra-abdominal and intra-pelvic abscesses. This organism may frequently be acquired by cross-infection from other patients and through contaminated medical equipments. Diseases cause by this organism can be treated with antibiotics such as gentamycin, vancomycin and daptomycin. Cautious use of antibiotics and implementation of infection control measures are ways of preventing this infection. Understanding the pathogenicity and antibiotics resistance of this organism will help in preventing it from causing nosocomial infection

Salwa Mohamed Ali Al-Fituri

Lab medicine, Microbiology, Libya

Title: Prevalence of Bacterial Meningitis and Their ANTIBIOTIC Sensitivity Patterns in Children Admitted To Hospital In Benghazi

Biography:

Abstract:

To explore the case management and disease burden of bacterial meningitis among children, admitted to Benghazi hospital from (January 2014 - December 2015), to study the trends of bacterial aetiology amongst cases of meningitis. and to isolate, identify and determine the antibiotic susceptibility patterns of pathogens associated with bacterial meningitis Cerebrospinal fluid (CSF) samples were collected from children admitted to children hospital units aged from few days up to 13 years old, and suspected to have bacterial meningitis, Doctor will almost always do a lumbar puncture. In this retrospective study, the data were collected from the laboratory records and statistical unit. A total of 6306 CSF children samples from clinically suspected case of meningitis were subjected to microbiologic unit. There were (400) cases clinically diagnostic as BM in children, 103 cases were found to be CSF bacterial culture positive and (77) were positive by gram stain (41%). Most of meningitis cases were found in male’s children 225 (56%) than females175 (44%). The ratio of male to female was (1.3-1). Fever was the most common presentation (90%) followed by vomiting (60%), poor oral intake (51%). The mortality rate was (7%), Coagulase negative staphylococcus was the most frequent pathogens isolated (55%)followed by E. coli (15.5%)then staph aureus (14.6), followed by streptococcus pneumonia (11.7%), Klebsiella pneumoniae (9.7%), Acinetobacter species, (5.8%), then Pseudomonas species, (4.9%), and among the seasonal effect the highest incidence in the study area were occurred on Augustus.

Andrzej Lange

Hirszfeld Institute of Immunology and Experimental Therapy

Title: Bone marrow mononuclear cells if locally injected may bring relief to patients with critical limb ischemia or osteoarthrosis due to the presence of subpopulations specifically addressing the main symptoms

Biography:

Prof. Andrzej Lange, MD, FRCP graduated with a medical degree with distinction from the Medical School in WrocÅ‚aw, Poland is a professor in the Institute of Immunology and Exp Therapy of the Polish Academy of Sciences and a founder and head of the Lower Silesian Center of Cellular Transplantation in WrocÅ‚aw. His international experience started in 1973 – 1974 as a Leverhulme Fellow in the Middlesex Hospital Medical School, London. He has been a visitor and lectured in a number of European and North American scientific institutions. Known from his activity in the field of bone marrow transplantation and regenerative medicine.

Prof. Lange was awarded with several scientific distinctions and served to a number of National and European institutions being also active in co-editing journals. He is an author and co-author of 249 scientific papers, in peer-reviewed journals with a cumulative IF of 270 in the years 1995–2017

Abstract:

In 2004 we started a project on revascularization of legs with critical ischemia (CLI). Eleven years later a similar approach was employed to improve vascularization of the femur with avascular necrosis (AVN). In both attempts a leukophoretic product resulting from the separation (Spectra, Optia) of 500 mL of the marrow was injected in small portions into the calf of legs with ischemia or into Kirschner wire drilled holes penetrating the head of the femur. In CLI patients employed procedure resulted in a prompt relief of pain followed by healing of the ulceration and significantly prolonged intervals between episodes of intermittent claudication. Forty six percent of patients benefited from the improvement. As to AVN, an improvement seen in 3 out of 5 patients was reported as pain relief and improvement in movement.

More recently instead of leukophoresis, a commercially available kit was used for the marrow manipulation. The cells were injected into the hip (7 cases) or the knee (12 cases). The effect was seen as soon as two weeks after the implantation as the relief of pain and improvement in movement.

A higher contribution of CD45-CD34- CD90+ and CD73+ cells to the implanted inoculum was beneficial for osteoarthritis treatment but not in improvement of vasculature (likely endothelial stem cells). In the inoculum used for regeneration two subpopulations of cells were identified having endothelial or mesenchymal cell markers. Notably the cells having an unique marker and that of stemness potential, were localized in the gate of small forward and side scatters.

In conclusion, the angiogenic and anti-inflammatory potential of BMMNC has been clinically documented, and the information that different subsets of the cellular inoculum contribute to the clinical outcome opens a new avenue for targeted therapy using subsets of stem cells for optimal application of treatment

Alain Chapel

Institute of Radiological Protection and Nuclear Safety, France

Title: Stem cell therapy for the treatment of severe tissue damage after radiation exposure

Biography:

For 25 years, he has been developing gene and cell therapy using non-human primates, immune-tolerant mice and rats to protect against the side effects of radiation. He collaborates with clinicians to develop strategies for treatment of patients after radiotherapy overexposures. He has participated in the first establishment of proof of concept of the therapeutic efficacy of Mesenchymal stem cells (MSCs) for the treatment of hematopoietic deficit, radiodermatitis and over dosages of radiotherapy. He has contributed to the first reported correction of deficient hematopoiesis in patients (graft failure and aplastic anemia) thanks to intravenous injection of MSCs restoring the bone marrow microenvironment, mandatory to sustain hematopoiesis after total body irradiation. He is scientific investigator of Clinical phase II trial evaluating the efficacy of systemic MSC injections for the treatment of severe and chronic radiotherapy-induced abdomino-pelvic complications refractory to standard therapy (NCT02814864Hirsch Index 26.

Abstract:

The late adverse effects of pelvic radiotherapy concern 5 to 10% of them, which could be life threatening. However, a clear medical consensus concerning the clinical management of such healthy tissue sequelae does not exist. Our group has demonstrated in preclinical animal models that systemic MSC injection is a promise approach for the medical management of gastrointestinal disorder after irradiation. We have shown that MSC migrate to damaged tissues and restore gut functions after irradiation.

The clinical status of four first patients suffering from severe pelvic side effects resulting from an over-dosage was improved following MSC injection in a compassional situation. A quantity of 2x106 - 6x106 MSC /kg were infused intravenously to the patients. Pain, hemorrhage, frequency of diarrheas and fistulisation as well as the lymphocyte subsets in peripheral blood were evaluated before MSC therapy and during the follow-up. Two patients revealed a substantiated clinical response for pain and hemorrhage after MSC therapy. In one patient pain reappeared after 6 months and again substantially responded on a second MSC infusion. A beginning fistulisation process could be stopped in one patient resulting in a stable remission for more than 3 years of follow-up. The frequency of painful diarrhea diminished from an average of 6/d to 3/d after the first and 2/d after the 2nd MSC injection in one patient. In all patients, prostate cancer remained in stable complete remission. A modulation of the lymphocyte subsets towards a regulatory pattern and diminution of activated T cells accompanies the clinical response in refractory irradiation-induced colitis. No toxicity occurred.

MSC therapy was safe and effective on pain, diarrhea, haemorrhage, inflammation, fibrosis and limited fistulisation. For patients with refractory chronic inflammatory and fistulising bowel diseases, systemic MSC injections represent a safe option for salvage therapy. A clinical phase II trial will start in 2018

Jae Seo Lee

Kyung Hee University, Republic of Korea

Title: Dual Stimuli-Responsive Degradable Quantum Dot-based Theranostic Agents targeting Breast Cancer

Biography:

Jaeseo Lee is a MS candidate at the Kyung Hee University, and she began researching in the Kwon lab in Jan of 2016. She has her expertise in tissue engineering and biomaterials. She is working to research drug delivery system using graphene quantum dots. She is also leading a project to develop drug delivery vehicles to treat specific breast cancer.

Abstract:

Graphene Quantum Dots (GQD) have been widely used for theragnosis for cancer disease. However, one of the current challenges is considerably difficult to release therapeutic agent to target site. In this study, we modified GQD that introduced herceptin through polyethylene glycol (PEG) and disulfide bond to induce active targeting for specific cells and control the drug release. The resulting of UV spectroscopy, Fluorescence, Fourier Transform Infrared showed that GQD-complex (GQD-comp) was conjugated successfully. The cell viability was presented that GQD-comp had a substantial effect on HER2-overexpressing breast cancer cells. In endosomal condition of low pH and high glutathione, doxorubicin was rapidly released from Doxorubicin loaded GQD-comp. Besides, this optical imaging by confocal laser scanning microscopy indicated to certainly confirm intracellular trafficking. In conclusion, GQD-comp had potential theragnostic agents to HER2-positive breast cancer cells.

Donghyun Lee

Kyung Hee University, Republic of Korea

Title: Poly(lactide-co-glycolide) nanofibrous membrane coated with gold-nanoparticles for guided bone regeneration

Biography:

Donghyun Lee is a Ph.D. candidate at the Kyung Hee University, and he began researching in the Kwon lab in May of 2013. He has his expertise in tissue engineering and biomaterials. He is working to research biological properties of gelatin hydrogel and gold nanoparticle with the goal of designing injectable material systems for bone regeneration. He is also leading a project to develop biomaterial to treat alveolar bone defects. He has published 4 scientific research papers as a first author and 18 papers as a co-author.

Abstract:

Poly(lactide-co-glycolide) (PLGA) is a biocompatible and biodegradable polymer that has been widely used in devices for tissue engineering and drug delivery applications. Gold nanoparticles (GNPs) have also been used as biomaterials and have been found to have a positive effect on bone formation. In this study, we synthesized thiol end-capped PLGA (PLGA-SH) and used it for binding GNPs. This PLGA was processed into a sheet form via electrospinning. GNPs with an approximate size of 30 nm were attached onto the PLGA-SH sheet surfaces (PLGA-GNPs). This membrane was characterized by thermogravimetric analysis, ultraviolet/visible spectrophotometry, field emission scanning electron microscopy, energy dispersive X-ray spectroscopy, X-ray photoelectron spectroscopy, and confocal laser scanning microscopy. Characterization results show that the GNPs are well attached on the PLGA-SH sheet and it is possible to control the GNPs load. Additionally, in-vitro results showed that PLGA-GNPs have good biocompatibility. They were also found to enhance osteogenic differentiation of human adipose derived stem cells. From these results, we have determined that the PLGA-GNP fibers can be useful as materials for bone regeneration and can also potentially serve as drug carriers.

Muhammad Uzair Rehman

United Medical & Dental College, Pakistan

Title: Evaluation of the effects of Platelet Rich Plasma and Stem Cell Based from Adult Adipose Tissue in regeneration of the spinal cord

Biography:

Abstract:

The purpose of study is to explore the efficacy and safety of platelet rich plasma (PRP) in the non-operative management of shoulder tendinopathy amongst individuals with spinal cord injury.

In this case in road traffic accidents that cause spinal injuries to the central nervous system, which increased morbidity and motility. The complications in patients were developed, resulting to a challenging problem for medicine. Platelet is naturally involved in haemostasis and tissue healing processes due to their content in growth factor and other bioactive molecules. Basic science and preclinical evidence supports the use of platelet derived growth factors as well as of PRP for enhancing reparatory processes in spinal cord injury.

In recent advancement in the field of regenerative medicine it was thought that this would grant a new approach to this problem, as it had proven beneficial in the repair of peripheral nerve injuries and their regeneration. This study was done to evaluate the effect of Platelet Rich Plasma (PRP) and its effect on the spinal cord post trauma. Subsequently, it was seen that PRP proved beneficial with marked positive effects in both muscle tone and muscle control and marked clinical improvement, although it can still be said that further research must be done in this field.

Clinical results about the use of PRP for bone, tendon, cartilage or spinal cord healing are encouraging and continue to accumulate in the recent years. Proteomic profiling and biomarker based PRP characterization have the potential of advancing the field of PRP application. High quality studies are awaited in order to enable clear cut therapeutic indications.

Marketa Benkova

University of Defence, Czech Republic

Title: Synthesis, antimicrobial efficacy and structure-activity relationships of three series of N-alkylammonium salts

Biography:

Marketa Benkova, née Pasdiorova, obtained her Master degree of Medical bioanalytics at the Faculty of Pharmacy in Hradec Kralove, Charles University in Prague in 2014. She worked for one year as a laboratory technician at the Department of Clinical Microbiology, University Hospital in Hradec Kralove. Currently, she is the postgraduate student of the Faculty of Military Health Sciences, University of Defence, and she also works as a researcher at Biomedical Research Center, University Hospital in Hradec Kralove. She is author or co-author of 15 publications in reputed journals.

Abstract:

Three series of N-alkylammonium salts (7a-c, 8a-b, 9a-b) based on quaternary ammonium compounds with a different length and type of carbon chain (C₁₂, C₁₄, C₁₆) were synthesized, characterized (EA, HRMS, NMR) and tested in vitro for antimicrobial (antibacterial, antifungal and antialgal) activity. Furthermore, the critical micelle concentration (CMC), capacity factors k and cytotoxicity were likewise measured to elucidate possible structure-activity relationships. The antimicrobial activity of the prepared compounds has been evaluated and compared. All compounds being tested proved high efficacy against both Gram-positive and Gram-negative bacterial strains, excluding the activity against multi-resistant Pseudomonas aeruginosa. Antifungal testing showed high activity of most compounds against fungal strains (yeasts and filamentous fungi) except of Aspergillus niger. The relationship between length of carbon chain and the efficiency has been observed. Series 7a-c proved high antialgal efficacy. Cell viability assay confirmed an expected trend that increasing carbon chain length results in higher cytotoxicity.

Sarita Manandhar

Tribhuvan University, Nepal

Title: Study of antibiotic susceptibility pattern and biofilm formation among staphylococci isolated from tertiary care hospital

Biography:

Abstract:

In-vitro persister assay is undergoing to study the causal link between persister cells and treatment failures in these isolates.